Mechanism study of long non-coding RNA DPP10-AS1 promotes lymph node metastasis in papillary thyroid carcinoma
10.3969/j.issn.1671-8348.2025.05.004
- VernacularTitle:长链非编码RNA DPP10-AS1促进甲状腺乳头状癌淋巴结转移的机制研究
- Author:
Jingli XUE
1
;
Ruijing LIU
;
Wen LIU
;
Yuqing WEI
;
Beibei JIANG
;
Yiqiang YIN
;
Peifeng LI
Author Information
1. 锦州医科大学中国人民解放军解放军联勤保障部队第九六〇医院研究生培养基地,济南 250031;中国人民解放军联勤保障部队第九六〇医院病理科,济南 250031
- Keywords:
papillary thyroid carcinoma;
lymph node metastasis;
lncRNA;
DPP10-AS1;
differential ex-pression
- From:
Chongqing Medicine
2025;54(5):1066-1073,1079
- CountryChina
- Language:Chinese
-
Abstract:
Objective To screen long non-coding RNA(lncRNA)associated with lymph node metasta-sis in papillary thyroid carcinoma(PTC)and verify its function in vitro,so as to provide a theoretical basis for elucidating the molecular mechanism of lymph node metastasis in PTC.Methods lncRNA+mRNA microar-ray was used to detect the differential expression of lncRNA and mRNA in PTC cancer tissues with and with-out lymph node metastasis.Real time fluorescence quantitative PCR(qPCR)verified target differential lncR-NAs.Lentivirus was used as vector to construct high and low lncRNA expression PTC cell lines.Cell counting kit-8(CCK-8),cell scratches assay,Transwell assay,cell clone formation assay were used to detect the effects of target lncRNA on proliferation,migration,invasion,clonal formation of PTC cells.Results Compared with 5 cases of PTC tissues without lymph node metastasis,gene chips detected 119 lncRNA and 53 mRNA expres-sion levels upregulated,while 263 lncRNA and 198 mRNA expression levels down regulated in 5 cases of PTC tissues with lymph node metastasis.Furthermore,21 lncRNAs were selected for validation in the original 10 PTC samples,and the results showed that,compared with PTC tissues without lymph node metastasis,lncR-NAs FLJ20444,DPP10-AS1 and ENST00000567197 were highly expressed in PTC tissues with lymph node metastasis,while uc021thd.1,LNC00944,ENST00000429730 and BLNK were lowly expressed(P＜0.05).In addition,qPCR results of another 30 fresh PTC tissues showed that compared with PTC tissues without lymph node metastasis,DPP10-AS1 was highly expressed and LNC00944 was lowly expressed in PTC tissues with lymph node metastasis(P＜0.05).Cell function experiments showed that the proliferation,migration,invasion,and colony formation abilities of PTC cells in the DPP10-AS1 high expression group were higher than those in the DPP10-AS1 low expression group,and the differences were statistically significant(P＜0.05).Conclusion lncRNA DPP10-AS1 may play a role in PTC metastasis through certain signaling pathways.
