Study on the mechanism of shikonin in the treatment of lipopolysaccharide-induced myocarditis by regulating the Gal-1/Nrf2 signaling pathway
10.3969/j.issn.1671-8348.2025.05.003
- VernacularTitle:紫草素调控Gal-1/Nrf2信号通路治疗LPS致心肌炎的机制研究
- Author:
Feng SHEN
1
;
Kongjie LU
Author Information
1. 浙江中医药大学第五临床医学院心血管内科,浙江湖州 313000;湖州市中心医院心血管内科,浙江湖州 313000
- Keywords:
shikonin;
myocarditis;
galectin-1;
nuclear factor-erythroid 2 related factor 2;
inflammation;
oxidative stress
- From:
Chongqing Medicine
2025;54(5):1059-1065
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of shikonin(SK)in the treatment of lipopolysaccha-ride(LPS)-induced myocarditis by regulating the galectin-1(Gal-1)/nuclear factor-erythroid 2 related factor 2(Nrf2)signaling pathway.Methods H9c2 cells were induced by LPS to establish an in vitro model of myocar-ditis,and were treated with different concentrations of SK for intervention.The cell counting kit-8(CCK-8)was used to detect cell viability,the TdT-mediated dUTP nick-end labeling(TUNEL)technique was used to detect cell apoptosis,the reactive oxygen species(ROS)kit was used to detect the ROS level,ELISA was used to detect the the levels of inflammatory indexes including interleukin(IL)-1β,tumor necrosis factor-α(TNF-α),IL-6 and the oxidative stress indexes including malondialdehyde(MDA)and superoxide dismutase(SOD).Polymerase chain reaction(PCR)and Western blot were used to detect the mRNA and protein ex-pressions of Gal-1 and Nrf2 respectively.Results Compared with the Ctrl group,the levels of ROS,IL-1β,TNF-α,IL-6 and MDA in the LPS group were increased,and the level of SOD was decreased,the differences were statistically significant(P＜0.05).Compared with the LPS group,the levels of ROS,IL-1β,TNF-α,IL-6 and MDA in the LPS+SK group were decreased,the level of SOD was increased,and the mRNA and protein expressions of Gal-1 were increased,the differences were statistically significant(P＜0.05);the levels of ROS,IL-1β,TNF-α,IL-6 and MDA in the LPS+Gal-1 group were decreased,and the level of SOD was in-creased,the differences were statistically significant(P＜0.05).Compared with the Ctrl group,the mRNA and protein expressions of Gal-1 in the LPS group were decreased,,the differences were statistically signifi-cant(P＜0.05);compared with the LPS group,the mRNA and protein expressions of Nrf2 in the LPS+Gal-1 group were increased,but those in the LPS+si-Gal-1 group were decreased,the differences were statistically significant(P＜0.05).Conclusion SK may exert anti-inflammatory and antioxidant stress effects by up-regu-lating the expression of Gal-1/Nrf2 pathway,and thus improve LPS-induced myocarditis.
