Study of the angiogenic effects and mechanisms of concentrated growth factor on human microvascular endothelial cells
10.3969/j.issn.1671-8348.2025.04.001
- VernacularTitle:浓缩生长因子促进人微血管内皮细胞血管形成的作用及机制研究
- Author:
Jiajia ZHANG
1
;
Shenglan SHANG
;
Wanbing LIU
;
Tao LI
Author Information
1. 中部战区总医院内分泌科,武汉 430070
- Keywords:
concentrated growth factor;
human microvascular endothelial cells;
angiogenesis;
phos-phoinositide 3-kinase/phospho-protein kinase B/mammalian target of rapamycin pathway
- From:
Chongqing Medicine
2025;54(4):801-805,812
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of concentrated growth factor(CGF)on angiogenesis in human microvascular endothelial cells(HMEC-1)and explore the underlying mechanisms.Methods HMEC-1 were divided into the control group,CGF group,and CGF+LY294002 group.Cell proliferation,migration,and tubule formation abilities were measured and compared using the Cell Counting Kit-8(CCK-8),Transwell assay,and tube formation assay,respectively.The protein expression levels of phosphoinositide 3-kinase(PI3K),phosphorylated PI3K(p-PI3K),phospho-protein kinase B(Akt),phosphorylated Akt(p-Akt),mam-malian target of rapamycin(mTOR),and phosphorylated mTOR(p-mTOR)were detected and compared by Western blot.Results Compared with the control group,the CGF group exhibited enhanced cell proliferation,migration,and tubule formation abilities(P＜0.05),along with increased expression levels of p-PI3K,p-Akt,and p-mTOR(P＜0.05).Compared with the CGF group,the CGF+LY294002 group demonstrated reduced cell proliferation,migration,and tubule formation abilities(P＜0.05),accompanied by decreased expression levels of p-PI3K,p-Akt,and p-mTOR(P＜0.05).Conclusion CGF promotes the proliferation,migration,and angiogenesis of HMEC-1 via the PI3K/mTOR/Akt pathway.
