HIV-1 pretreatment drug resistance and molecular transmission network characteristics in Yubei District,Chongqing
10.3969/j.issn.1671-8348.2025.03.028
- VernacularTitle:重庆市渝北区HIV-1治疗前耐药及分子传播网络特征分析
- Author:
Difei LI
1
;
Ying XU
;
Mao YE
;
Xin HUANG
;
Xuemei MA
;
Yi JIN
;
Songsong SUN
;
Jinping XIONG
;
Hui LIU
;
Guohui WU
Author Information
1. 重庆市渝北区疾病预防控制中心,重庆 401120
- Keywords:
human immunodeficiency virus-1;
pretreatment drug resistance;
molecular transmission network;
Yubei District of Chongqing city
- From:
Chongqing Medicine
2025;54(3):719-724,730
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the characteristics of HIV-1 pretreatment drug resistance(PDR)and molecular transmission networks in Yubei District,Chongqing,providing evidence for targeted interventions.Methods Using a cross-sectional design,plasma samples were collected from HIV/AIDS patients receiving antiretroviral therapy(ART)in Yubei District from January 2022 to December 2023.Pol gene fragments were extracted and amplified for HIV-1 genotyping and drug resistance analysis.Molecular transmission networks were constructed based on genetic distance calculations.Results Among 478 HIV-1 pol sequences,eight geno-types were identified:with CRF07_BC(60.4%,289/478),CRF08_BC(15.5%,74/478),CRF01_AE(11.7%,56/478),and CRF85_BC(5.9%,28/478).The overall PDR rate was 6.3%(30/478),with resistance to nucleoside reverse transcriptase inhibitors(NRTIs)and non-nucleoside reverse transcriptase inhibitors(NNRTIs)at 1.7%(8/478)and 5.2%(25/478),respectively.No protease inhibitor(PI)resistance was de-tected.The molecular network included 177 cases(37.0%network entry rate),forming 53 clusters with 198 connections.Cluster sizes ranged from 2 to 17 nodes,and 75.3%(149/198)of connections were associated with five subdistricts/towns:Shuanglonghu Street,Huixing Street,Luoqi Town,Gulu Town,and Baoshenghu Street.Conclusion HIV-1 genotypes in Yubei District exhibit diversity and complexity,with moderate PDR prevalence.Regional clustering of transmission networks suggests the need for enhanced molecular surveil-lance and targeted interventions based on analytical findings.
