Expression of lncRNA KCNQ1OT1 in renal tissues of patients with idiopathic membranous nephropathy and its correlation with number of podocytes and clinical indexes
10.3969/j.issn.1671-8348.2025.02.022
- VernacularTitle:lncRNA KCNQ1OT1在IMN患者肾组织中的表达及与足细胞数和临床指标的相关性分析
- Author:
Jia FAN
1
;
Jing LEI
;
Nan SUN
;
Ping HE
Author Information
1. 中国医科大学附属盛京医院:肾内科,沈阳 110004;中国医科大学附属盛京医院:消化科,沈阳 110004
- Keywords:
idiopathic membranous nephropathy;
lncRNA;
KCNQ1OT1;
podocytes number;
serum al-bumin
- From:
Chongqing Medicine
2025;54(2):418-424
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of long non-coding RNA(lncRNA)and long potassium voltage-gated channel subfamily Q member 1 overlapping transcript 1(KCNQ1OT1)in renal tissues of the patients with idiopathic membranous nephropathy(IMN)and its correlation with podocytes number and clini-cal indicators.Methods A total of 24 patients with diagnosed IMN in the nephrology department of this hos-pital from February to June 2022 were selected as the IMN group,and 22 patients with nephrectomy due to re-nal cancer in the urologic surgery department during the same period were selected as the control group.The expression of lncRNA KCNQ1OT1 was detected by fluorescence in situ hybridization(FISH)and real-time quantitative PCR(qPCR).Wilms tumor gene 1(WT1)was used to label podocyte nuclei,and the expression level of WT1 was detected by immunohistochemical staining.The clinical data of the patients were collected to study the relationship between lncRNA KCNQ1OT1 expression in kidney tissue with the clinical indicators such as podocytes number,serum albumin and 24 h urine protein quantification in IMN patients.Results The mean gray value of lncRNA KCNQ1OT1expression green channel in the IMN group was 1.76±0.27 in the FISH detection,which was higher than 1.00±0.14 in the control group,and the difference was statistically significant(t=11.911,P<0.05).The expression level of lncRNA KCNQ1OT1 in the IMN group was 2.40±0.17 by qPCR,which was higher than 1.00±0.03 in the control group,and the difference was statistically sig-nificant(t=13.653,P<0.05).The expression level of WT1 in the IMN group was 13.27±5.18,which was lower than 28.79±5.58 in the control group,and the difference was statistically significant(t=-9.777,P<0.05).The expression level of lncRNA KCNQ1OT1 in kidney tissue of IMN patients was negatively correla-ted with the podocytes number and serum albumin(P<0.05),positively correlated with 24 h urine protein quantification(P<0.05),but had no significant correlation with serum creatinine,urea,anti-PLA2R antibody and eGFR(P>0.05).Conclusion KCNQ1OT1 of lncRNA may be related with the occurrence and develop-ment of IMN,and is expected to be a potential target for the treatment of IMN.
