Establishment and application of MALDI-TOF MS technique for detecting HPA-29-35w
10.3969/j.issn.1671-8348.2025.01.019
- VernacularTitle:MALDI-TOF MS检测HPA-29-35w技术的建立及应用
- Author:
Jierun CHEN
1
;
Yan ZHOU
;
Lilan LI
;
Zhoulin ZHONG
;
Lihong JIANG
;
Tingting MA
;
Hengcong LI
;
Qiuhong MO
Author Information
1. 南宁中心血站/南宁输血医学研究所,南宁 530003
- Keywords:
matrix-assisted laser desorption ionization-time or flight mass spectrometry;
human plate-let antigen;
genotyping;
gene polymorphsim
- From:
Chongqing Medicine
2025;54(1):101-107,113
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the genetic typing detection technique of matrix-assisted laser de-sorption ionization-time or flight mass spectrometry(MALDI-TOF MS),and to apply it to investigate the polymorphism of the human platelet antigen(HPA)-29-35w low-frequency gene in blood donor population from Guangxi area.Methods The RS number of 7 target gene mutation sites in dbSNP and 21 primers were designed by using Assay Design Suite(ADS)of MassARRAY platform online primer design tool.Seven over-expression vectors inserting into HPA-29-35bb mutant sequence were constructed.Twenty-nine blood donors were randomly selected among the blood donors team in Guangxi area.The samples and vectors conducted the iPLEX Pro multiple genotyping analysis and mass spectrometric detection.Meanwhile,the above samples were sequenced and the sequencing results conducted the comparison validation with the mass spectrometric detec-tion results,then the MALDI-TOF MS genotyping detection technology was established.Then adopting this technology conducted the platelet antigen HPA-29-35W genotyping detection and polymorphism analysis in the samples from 588 blood donors in Guangxi area.Results The MALDI-TOF MS HPA genotyping detec-tion results were consistent with the sequencing results.The HPA-29-35w genotyping results showed that theHPA-29-35w genotype of 588 blood donors in Guangxi area was aa homozygote.Conclusion The genotype detection method of MALDI-TOF MS for HPA-29-35w is successfully established and applied to the screening of HPA-29-35w gene among the blood donor population in Guangxi area.
