Analysis on mtDNA variation rate of elderly people at different age groups in a"regional longevity"gathering area in Chongqing
10.3969/j.issn.1671-8348.2025.01.009
- VernacularTitle:重庆某"区域长寿"聚集区不同年龄段老年男性的mtDNA变异率分析
- Author:
Xiaocang ZOU
1
;
Rui WANG
;
Weishuai WANG
;
Yongjun LUO
Author Information
1. 中国人民解放军陆军军医大学陆军卫勤训练基地军事医学地理学教研室,重庆 400038;中国人民解放军联勤保障部队第941医院康复医学科,西宁 810000
- Keywords:
mitochondrial DNA;
gene;
variation;
living habit
- From:
Chongqing Medicine
2025;54(1):46-51
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the differences in living habit and blood biochemical indicators of male elderly people at different age groups in a"regional longevity"gathering area in Chongqing and the high fre-quency variation of mitochondrial DNA(mtDNA)in the elderly people(≥90 years old).Methods The male elderly people aged ≥60 years old in a"regional longevity"gathering area in Chongqing were randomly select-ed as the study subjects and divided into the group A(60-＜70 years old),group B(70-＜80 years old),group C(80-＜90 years old)and group D(≥90 years old).The differences of vegetable and fruit consump-tion,dairy bean consumption,outdoor activities,sleep time and blood biochemical indexes were compared a-mong different age groups.Then peripheral blood in the above subjects was collected for extracting mtDNA and sequencing.The meaningful mtDNA mutations between the various groups and group D were found out by the bioinformatics analysis.Results There were no statistically significant differences in consumption of vegetables and fruits,consumption of dairy beans,outdoor activities and sleep duration among different age groups(P＞0.05).There were statistically significant differences in erythrocyte and creatinine of blood bio-chemical indexes among the partial groups(P＜0.05).The mitochondria mutation analysis found 4 105 mtD-NA mutation sites,mainly single base mutation,the most common mutation was T＞C(22.57％),followed by C＞T(19.82％)and A＞G(19.36％).The average mutation rate of mtDNA in the group D was the high-est(8.392％),in which 3 mtDNA variants were found to have significant differences between different age groups and group D(P＜0.1),including mtDNA5396-5411CAACGTAAA＞C,mtDNA5414A＞T and mtD-NA5417G＞T.Conclusion There was no significant difference in the living habits of male elderly people of different ages.The mutation rate of mtDNA in the advanced age group was the highest.mtDNA5396-5411CAACGTAAA＞C,mtDNA5414A＞T and mtDNA5417G＞T variations may be related to"regional lon-gevity".
