Study on effect of andrographolide for inhibiting proliferation of colon cancer cells by up-regulating BMP9
10.3969/j.issn.1671-8348.2025.01.005
- VernacularTitle:穿心莲内酯通过上调BMP9抑制结肠癌细胞增殖作用研究
- Author:
Shiyu WANG
1
;
Xiaoli ZHOU
;
Yanmei LI
;
Baicheng HE
;
Jun SHEN
;
Zhenhua CHEN
Author Information
1. 重庆护理职业学院,重庆 402763;重庆医科大学药学院药理学教研室,重庆 400016;重庆市生物化学与分子药理学重点实验室,重庆 400016
- Keywords:
andrographolide;
colon cancer;
proliferation inhibition;
bone morphogenetic protein 9;
PI3K/Akt
- From:
Chongqing Medicine
2025;54(1):24-30
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the related molecular mechanism of andrographolide(AGP)in inhibi-ting the proliferation of human colon cancer LoVo cells and promoting their apoptosis.Methods The LoVo cells were set up as the control group and experimental group.The cells were treated with different concentra-tions of AGP(0,20,40,80 μmol/L).The cell viability was detected by CCK-8,the cellular cycle and apoptosis were detected by flow cytometery,and Western blot was used to detect protein level of PCNA,Bad and Bcl-2,and the effect of AGP of BMP9 overexpression or silencing AGP on PCNA,Bad and Bcl-2 protein levels;West-ern blot was used to analyze the effect of AGP on PTEN,Akt1/2/3 and p-Akt1/2/3 levels.Results AGP could inhibit the proliferation of LoVo cells,promoted their apoptosis and increased the BMP9 expression lev-el.The BMP9 overexpression could enhance the effect of AGP for inhibiting the proliferation and promoting the apoptosis,silencing BMP9 could weaken the above effects of AGP(P＜0.05).AGP decreased the expres-sion level of p-Akt1/2/3 in the LoVo cells and increased the PTEN expression level(P＜0.05).BMP9 over-expression enhanced AGP and decreased the p-Akt 1/2/3 expression level in the LoVo cells,and silencing BMP9 could weaken the above effects of AGP(P＜0.05).Conclusion AGP could inhibit the proliferation of human colon cancer LoVo cells,its mechanism may be related with up-regulating BMP9 expression,thus in-crease PTEN protein level and inhibit P13K/Akt signal.
