Effect of NK cells on proliferation of colorectal cancer cells
10.3969/j.issn.1671-8348.2025.01.004
- VernacularTitle:NK细胞对结直肠癌细胞增殖的影响
- Author:
Subing LIU
1
;
Ziyu YE
;
Yanfang LIANG
;
Jincheng ZENG
Author Information
1. 东莞市石排医院检验科,广东东莞 523330;广东医科大学东莞市医学活性分子开发与转化重点实验室,广东东莞 523808
- Keywords:
NK cells;
colorectal cancer cells;
effect-target ratio;
cell proliferation;
adoptive cell immuno-therapy
- From:
Chongqing Medicine
2025;54(1):18-23
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of NK cells on the proliferation of four kinds of colorec-tal cancer(CRC)lines,and to explore the feasibility of adoptive NK cell immunotherapy in the treatment of CRC so as to provide an experimental basis for the diagnosis and treatment of CRC.Methods Peripheral blood mononuclear cells were isolated by the Ficoll density gradient centrifuge method,which were in vitro in-duced to activate as the NK cells and amplified.The CCK-8 method was used to detect the effect of NK cells on the proliferation of CRC cell lines RKO,HCT15,HCT116 and LoVo.The inhibition rate of NK cells on CRC cell lines was statistically analyzed and compared.Results The inhibitory rate of NK cells against the same target cells was significantly different at different effect target ratios(P＜0.05).Under different num-ber of target cells(5 × 103 vs.1 × 104),the inhibitory rate of NK cells against RKO(effect-target ratio 0.4∶1),HCT15(effect-target ratio 0.4∶1 and 0.2∶1),HCT116(effect-target ratio 3.2∶1,1.6∶1,0.8∶1,0.4∶1 and 0.2∶1)and LoVo(effect-target ratio 1.6∶1,0.8∶1,0.4∶1,0.2∶1 and 0.1∶1)were significantly different(P＜0.05),while no statistical differences were found among other groups(P＞0.05).The effect-target ratio corresponding to the maximum inhibitory rate of NK cells against four CRC cell lines was 12.8∶1 under different target cell numbers.Conclusion Adoptive NK cell immunotherapy has an impor-tant significance for the early intervention and treatment of CRC,moreover 12.8∶1 may be a safe and effec-tive effect-target ratio.
