Mechanism of epigallocatechin-3-gallate for inhibiting Aβ generation in N2a/APP695swe cells via regulation of LXRβ-RXRa-ABCA1 pathway
10.3969/j.issn.1671-8348.2025.01.003
- VernacularTitle:EGCG通过调控LXRβ-RXRα-ABCA1通路抑制N2a/APP695swe细胞内Aβ生成的机制研究
- Author:
Xuefei WANG
1
;
Hui WANG
;
Fulan YANG
;
Na YANG
;
Liu YANG
Author Information
1. 重庆市急救医疗中心/重庆大学附属中心医院神经内科,重庆 400010
- Keywords:
epigallocatechin-3-gallate;
β-amyloid;
LXRβ-RXRα-ABCA1 pathway;
caveolin-1;
BACE1
- From:
Chongqing Medicine
2025;54(1):12-17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study its effect and mechanism of epigallocatechin-3-gallate(EGCG)on the generation of Aβ42 in N2a/APP695swe cells.Methods The N2a/APP695swe cells were cultured in vitro and treated with different concentrations of EGCG(or in combination with LXRβ antagonist GSK2033).The DM-SO group and wild type N2a/wt group were set.The cellular survival rate was detected by the MTT assay;ELISA was used to detect the Aβ42 level;Western blot was used to detect the expression levels of LXRβ,RXRα,ABCA1,caveolin-1 and BACE1 proteins.Results The cellular survival rate and Aβ42 level in the 20,40 μmol/L EGCG cells groups were significantly improved compared with the other groups(P＜0.05),more-over which showed the concentration dependence(P＜0.05).After 20 μmol/L EGCG action,the expression levels of LXRβ,RXRα and ABCA1 protein were increased,the expression levels of caveolin-1 and BACE1 pro-tein were significantly decreased,and the differences were statistically significant(P＜0.05);after treating the cells by combining with GSK2033,the expression levels of LXRβ,RXRα and ABCA1 protein were significantly decreased and caveolin-1 and BACE1 protein expression levels were significantly increased(P＜0.05).Conclu-sion The generation of Aβ42 in N2a/APP695swe cells could be inhibited by EGCG,thus which inhibits the cellular proliferation,and its mechanism may be related to EGCG activating the LXRβ-RXRα-ABCA1 path-way,and then inhibiting the expression of caveolin-1 and BACE1.
