Expression and significance of ChREBP network members and TUG1 in intrauterine adhesion
10.3969/j.issn.1671-8348.2025.01.002
- VernacularTitle:ChREBP网络成员及TUG1在宫腔粘连中的表达及意义
- Author:
Lingjing ZHU
1
;
Mingqing CHEN
;
Zixuan YAN
;
Xuelan YAN
;
Ying AI
Author Information
1. 昆明理工大学医学院/云南省第一人民医院,昆明 650500
- Keywords:
intrauterine adhesion;
taurine-upregulated gene 1;
carbohydrate response element-binding protein;
mechanic uterine curettage;
lipopolysaccharide infection
- From:
Chongqing Medicine
2025;54(1):7-11,17
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression and significance of carbohydrate response element-binding protein(ChREBP)network members(ChREBP,Mlx,Max,Mxd1,HDAC1)and taurine-upregulated gene 1(TUG1)in intrauterine adhesion(IUA).Methods Ten female SD rats with 8 weeks old were selected as the study objects.The mechanical curettage plus lipopolysaccharide infection were used to construct the IUA rat model as the IUA group(n=5)and the uterine cavity of the control group did not perform any oper-ation(n=5);HE and Masson staining were respectively used to assess the number of endometrial glands and the degree of fibrosis;Western blot was used to detect the expression level of ChREBP network members in the nucleus and cytoplasm;RT-qRCR was used to detect the expression level of TUG1.Results Compared with the control group,the number of endometrial glands in the IUA group was decreased(P＜0.05),the percentage of the fibrotic area was increased(P＜0.05),the protein expression level of ChREBP network members in the nucleus was down-regulated,the protein expression level of ChREBP network members in the cytoplasm was up-regulated and the mRNA relative expression level of TUG1 was up-regulated(P＜0.05).Conclusion Blocked nuclear translocation of ChREBP and reduced the recruitment of its downstream network mem-bers may lead to attenuate the repressive effect on TUG1 transcription,thereby promoting IUA occurrence.
