Construction of teaching experimental conditions for determination of drug antagonism parameters with bullfrog as experimental object
10.3969/j.issn.1671-8348.2024.23.003
- VernacularTitle:以牛蛙为实验对象药物拮抗参数测定的教学实验条件构建
- Author:
You JIN
1
;
Hui LIU
;
Fang YANG
;
Fang WANG
;
Shangbin YU
Author Information
1. 华中科技大学国家级基础医学实验教学示范中心,武汉 430030
- Keywords:
bullfrog;
acetylcholine;
dose-effect curve;
tubocurarine;
antagonistic parameter pA2
- From:
Chongqing Medicine
2024;53(23):3535-3539
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the application of bullfrog as the experimental object in pharmacoki-netic experiment drug dose-effect curve drawing and competitive antagonist pA2 determination.Methods The rectus abdominis muscle of bullfrog served as the sample.The semi-logarithmic molal concentration accumula-tion method was used.The effect of competitive antagonist-tubocurarine on the contraction action of acetyl-choline(ACh)agonist was observed.Results The rectus abdominis of bullfrog(regardless of gender)weighed 200-250 g served as the sample.The ACh solution of 1.3 × 10-4-1.3 × 10-1 mol/L series concen-trations could cause the contraction reaction of most specimens when adding the second and third doses,more-over which could make the contraction of the sample to reach the maximum effect;0.4X 10-3 mol/L tubocura-rine solution could effectively antagonize the effect of Ach;in addition,the contraction effect generated the specimen was highly correlated with the effect of bullfrog or toad as the experiment animal.Conclusion The determination conditions of bullfrog antagonistic parameters could meet the experimental requirements.
