Protective effect of human amniotic mesenchymal stem cells on pulmonary microvascular endothelial cell injury induced by pine sawdust smoke solution in rats
10.3969/j.issn.1671-8348.2024.23.002
- VernacularTitle:人羊膜间充质干细胞对松木屑烟雾溶液致大鼠肺微血管内皮细胞损伤的保护作用
- Author:
Qiu ZHAN
1
;
Pei CUI
;
Xiulian ZHU
;
Rongsheng LI
;
Fengji MENG
;
Fujun ZHU
;
Fuwang YANG
;
Yalin TONG
;
Haiming XIN
Author Information
1. 中国人民解放军联勤保障部队第九二四医院动物实验室,广西桂林 541002
- Keywords:
smoke inhalation injury;
acute lung injury;
human amniotic mesenchymal stem cells;
pul-monary microvascular endothelial cells
- From:
Chongqing Medicine
2024;53(23):3527-3534
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective effect of human amniotic mesenchymal stem cells(hAMSCs)on pulmonary microvascular endothelial cell(PMVECs)through observing the effects of prolifer-ation,apoptosis and inflammatory response after PMVECs injury induced by pine sawdust smoke solution.Methods HAMSCs and rat PMVECs were isolated and cultured.The flow cytometry and immunofluores-cence were used to identify hAMSCs and PMVECs respectively.The experimental grouping:control group(normal cultured PMVECs),smoke group(PMVECs injury induced by pine sawdust smoke solution),smoke+hAMSCs group(after PMVECs was injured by smoke solution,hAMSCs and PMVECs were co-cul-tured in Transwell culture system).The proliferative activity of PMVECs after co-culture for 12,24 h was measured by cell counting kit-8(CCK-8),the apoptosis of PMVECs was measured by flow cytometry,and the ex-pression levels of TNF-α and IL-6 were detected by enzyme-linked immunosorbent assay(ELISA).Results hAMSCs and PMVECs were successfully isolated and cultured,and the hAMSCs surface markers CD105(95.4％),CD73(99.8％)and CD90(99.8％)were identified as strongly positive expression,while CD34,CD45,CD14,CD19 and HLA-DR were weakly expressed(1.96％in total).The vascular endothelial cell mark-er CD34 in PMVECs was positively expressed,moreover its combination with aggulutinin BSI was also posi-tive.At the observation time point of 12,24 h co-culture,compared with the Control group,the proliferation activity of PMVECs in the Smoke group was inhibited(P＜0.05),and the cellular apoptosis was increased(P＜0.05),the TNF-α and IL-6 expression levels were up-regulated(P＜0.05);the phenomena of PMVECs proliferation activity inhibition,apoptosis increase and inflammatory factor expression level up-regulation in the Smoke+hAMSCs group were reversed compared with the Smoke group(P＜0.05).Conclusion After PMVECs are injured by smoke solution,hAMSCs could decrease the PMVECs inflammatory factors expres-sion,promote its proliferation activity and inhibit its apoptosis,thus play the protective effect on PMVECs.
