Intratumoral Gemella haemolysans promotes neoadjuvant chemoimmuno-therapy efficacy in esophageal squamous cell carcinoma-bearing mice
10.13406/j.cnki.cyxb.003919
- VernacularTitle:瘤内Gemella haemolysans对增强食管鳞状细胞癌荷瘤小鼠新辅助化学免疫治疗疗效的研究
- Author:
Qinghua SONG
1
;
Ruijin LU
;
Yutang HUANG
;
Li CHEN
;
Lanxiang WU
Author Information
1. 重庆医科大学附属第一医院胸心外科,重庆 400016
- Keywords:
esophageal squamous cell carcinoma;
Gemella hae-molysans;
neoadjuvant chemoimmunotherapy
- From:
Journal of Chongqing Medical University
2025;50(10):1345-1352
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether intratumoral Gemella haemolysans(G.haemolysans)enhances the efficacy of neoadjuvant chemoimmunotherapy(NACI)in esophageal squamous cell carcinoma(ESCC)-bearing mice and the underlying mechanism.Methods:Twenty-five 4-week-old female C57BL/6 mice were randomized into five groups(n=5 per group):negative control(NC)group,chemo-therapy group,G.haemolysans+chemotherapy group,NACI group,and G.haemolysans+NACI group.A germ-free mouse model was established in each group by administering antibiotic cocktail for 2 weeks.On day 0,ESCC-bearing mouse models were built through subcutaneous inoculation of either murine esophageal cancer cell line AKR or G.haemolysans-AKR cells.On day 7 when tumors formed under the skin,drugs were injected into the abdomen thrice weekly.On day 28 when the treatments were finished,the mice were sacrificed to harvest their tumors,and then the tumors were fixed in 4%paraformaldehyde.We located and quantified G.haemolysans within the tumors through fluorescence in situ hybridization,and characterized immune cell composition in the tumor immune microen-vironment(TIME)of the mice treated with NACI through multiplex immunohistochemistry and flow cytometry.Results:G.haemolysans significantly strengthened the efficacy of NACI in ESCC(P<0.05),whereas no chemosensitizing effects were observed on chemo-therapy in ESCC-bearing mice.G.haemolysans significantly en-hanced natural killer(NK)cell infiltration within the TIME(P<0.01).Conclusion:G.haemolysans improves NACI efficacy in ESCC-bearing mice by increasing NK cell recruitment in the TIME.
