Effects of mesoporous bioactive glass on the composition and function of dental plaque biofilm
10.13406/j.cnki.cyxb.003848
- VernacularTitle:介孔生物活性玻璃对牙菌斑生物膜组成及功能的调控作用研究
- Author:
Yu CHEN
1
;
Yufei WANG
;
Linglin ZHANG
Author Information
1. 口腔疾病防治全国重点实验室/国家口腔疾病临床医学研究中心/四川大学华西口腔医院牙体牙髓病科,成都 610041
- Keywords:
dental plaque;
biofilm;
nanoparticle;
metatranscrip-tome;
tricarboxylic acid cycle
- From:
Journal of Chongqing Medical University
2025;50(9):1261-1270
- CountryChina
- Language:Chinese
-
Abstract:
Objective:The composition and function of dental plaque biofilm are susceptible to alterations,potentially leading to vari-ous oral diseases.Although mesoporous bioactive glass(MBG)has been widely used,how it affects the microbial composition and bio-logical function of dental plaque biofilm remains unclear.This study aims to investigate the effects of MBG on the composition and bio-logical function of viable bacteria in dental plaque in vitro.Methods:At first,MBG was synthesized and characterized.Subsequently,the MBG was applied to dental plaque biofilm in vitro for a short term.The impact on biofilm biomass was assessed through colony-forming unit(CFU)counting.MBG-treated samples were collected for metatranscriptomic analyses of species composition and differen-tially expressed genes.Finally,reverse transcription quantitative polymerase chain reaction(RT-qPCR)was performed to validate the expression of core DEGs.Results:The results of CFU counting showed that short-term MBG treatment reduced dental plaque biofilm biomass in vitro by 44.25%.The results of metatranscriptomics showed that MBG significantly changed the species composition of den-tal plaque biofilm,where commensal Streptococci and Klebsiella were predominant.The function of carbohydrate metabolism was up-regulated,and the up-regulated genes were mainly enriched in the tricarboxylic acid(TCA)cycle.The results of RT-qPCR showed that a total of 9 genes associated with the TCA cycle were up-regulated.Conclusion:MBG can affect the species composition and enhance the carbohydrate metabolism of dental plaque biofilm in vi-tro,providing a new theoretical basis for the application of MBG in the oral field.
