Effect of polystyrene microplastics combined with high-fat treatment on vascular endothelial cells
10.13406/j.cnki.cyxb.003734
- VernacularTitle:聚苯乙烯微塑料联合高脂处理对血管内皮细胞的影响
- Author:
Jing WANG
1
;
Jiani DIAO
;
Jing LONG
;
Yuguang HE
;
Lipin TAN
;
Xuemei CHEN
;
Fangfang LI
;
Junlin HE
;
Yingxiong WANG
;
Rufei GAO
;
Weike LI
Author Information
1. 重庆医科大学公共卫生学院生殖与发育教育部国际合作联合实验室,重庆 400016
- Keywords:
polystyrene microplastics;
high-fat;
human umbilical vein endothelial cells;
tube-forming ability
- From:
Journal of Chongqing Medical University
2025;50(7):969-976
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of polystyrene microplastics(PS-MPs)combined with high-fat treatment on vascular endothelial cells.Methods:Human umbilical vein endothelial cells(HUVECs)were cultured in the DMEM medium containing 5%fe-tal bovine serum.HUVECs were treated with conventional culture,high-fat treatment,and PS-MPs combined with high-fat treatment.The experiment was conducted in the three groups of control group,high-fat treatment group and PS-MPs+high-fat treatment group.CCK-8 assay was used to measure cell viability,F-actin staining was used to observe cell morphological changes,and flow cytometry,scratch assay,and tube formation assay were used to measure the apoptosis,migration,and tube-forming ability of cells.Results:After HUVECs were exposed to the high-fat environment,there was a significant reduction in cell viability,shrinkage of cells,a signifi-cant increase in cell apoptosis,and significant reductions in cell migration and tube-forming ability.Compared with the high-fat treat-ment group,there were no significant changes in cell viability,cell morphology,cell apoptosis,and cell migration ability after PS-MPs combined with high-fat treatment,but the tube-forming ability of cells was further impaired.Conclusion:High-fat treatment will affect cell viability,change cell morphology,and damage vascular endothelial cell function,and PS-MPs combined with high-fat treat-ment can aggravate the damage of vascular endothelial cell function.
