Role of dendritic cell membrane microbubbles in the activation of T cells and the killing of colorectal cancer cells
10.13406/j.cnki.cyxb.003821
- VernacularTitle:树突状细胞膜微泡在T细胞激活与结直肠癌细胞杀伤中的作用研究
- Author:
Han XU
1
;
Liang ZHANG
;
Xiaojing LENG
;
Shujin YAN
;
Hua PANG
Author Information
1. 重庆医科大学附属第一医院核医学科,重庆 400042
- Keywords:
dendritic cell;
immunotherapy;
microbubbles;
cell membrane;
dendritic cell vaccine
- From:
Journal of Chongqing Medical University
2025;50(6):750-757
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To design and evaluate a cell membrane vaccine strategy based on dendritic cell membrane microbubbles(DCM@MBs),and to explore its potential application in tumor immunotherapy,especially the immune-specific killing of tumor cells through the activation of T cells.Methods:At first,tumor cell membrane proteins were extracted and dendritic cells(DCs)were acti-vated to confirm that tumor antigens could effectively stimulate the maturation of immature DCs.Mature DC membranes were then mixed with lipids to prepare DCM@MBs,which were characterized for morphology,size,and protein composition by confocal laser scanning microscopy and sodium dodecyl sulfate-polyacrylamide gel electrophoresis.Finally,in vitro co-culture experiments were con-ducted to assess the effect of DCM@MBs on the activation of T cells and their ability for specific killing of tumor cells.Results:In the in vitro DC activation experiment,after stimulation with tumor cell membrane proteins,the 25 μg/mL group had a significant increase in the expression level of MHC class Ⅱ molecule(25.167%±1.203%)on the surface of immature DCs compared with the control group(P<0.001),and DCM@MBs presented with microbubbles encapsulated by red cell membranes,with uniform dispersion and a size of 1-5 μm.In the in vitro co-culture experiment,the amount of breast cancer cells(9.893±0.341)%.Conclusion:The DCM@MBs strategy proposed in this study shows significant potential in tu-mor immunotherapy and can effectively activate T cells and specifically kill and eliminate tumor cells,which provides new ideas for tu-mor immunotherapy.
