Construction of a mouse model of house dust mite-induced allergic asthma with cognitive impairment
10.13406/j.cnki.cyxb.003689
- VernacularTitle:屋尘螨诱导过敏性哮喘伴认知功能障碍小鼠模型的构建
- Author:
Qiang FU
1
;
Yiling KE
;
Guiqiong HE
;
Huarong YU
Author Information
1. 重庆医科大学神经科学研究中心,重庆 400016
- Keywords:
allergic asthma;
cognitive impairment;
house dust mite;
animal model;
mouse
- From:
Journal of Chongqing Medical University
2025;50(5):649-656
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To construct a mouse model of house dust mite(HDM)-induced allergic asthma with cognitive impairment.Methods:C57 BL/6 mice aged 6-8 weeks were used as the study subjects.HDMs were used intranasally to induce allergic asthma in mice(HDM group).Simultaneously,a normal saline control group(NS group)and a blank control group(CN group)were established.At week 2 and month 1 of model construction,cognitive ability was tested using Barnes maze;pulmonary function was determined by nebulization with acetyl-β-methylcholine chloride solution,and pathological changes in lung and brain tissues were evaluated using hematoxylin-eosin staining,immunohistochemistry,and Western blotting.Results:At week 2,there were no significant differences in the enhanced pause(Penh),inflammatory cell infiltration around the bronchi,time and frequency of mice finding escape boxes,and changes in mature neuronal marker neuronal nuclei antigen(NeuN)and synaptophysin in the brain in the HDM group compared with the CN and NS groups;no neuroinflammation occurred in the brain.At month 1,compared with the CN and NS groups,the HDM group showed a significant increase in Penh and a large amount of inflammatory cell infiltration around the bronchi;the time of mice finding escape boxes significantly increased(F=19.600,P<0.001),and the frequency significantly decreased(F=10.150,P=0.002);the NeuN-positive area in the hippocampus of mice significantly decreased(F=6.449,P=0.012),and synaptophysin was significantly reduced(F=16.200,P=0.004);activation of microglial cells and neuroinflammation occurred.Conclusion:This study successfully constructed a mouse model of HDM-induced allergic asthma with cognitive impairment,providing a good animal model for exploring the mechanism of cognitive impairment caused by allergic asthma and drug inter-vention research.
