Application of platelet-rich plasma combined with Xianling Gubao Capsule in treatment of rats with knee osteoarthritis
10.13406/j.cnki.cyxb.003811
- VernacularTitle:富血小板血浆联合仙灵骨葆胶囊治疗大鼠膝骨关节炎的应用研究
- Author:
Zhenhua CAI
1
;
Muzhou XIAO
;
Heng LUO
;
Zhilan WU
;
Li LI
;
Cong LÜ
Author Information
1. 广州中医药大学第一附属医院/广东省中医临床研究所输血科,广州 510405
- Keywords:
osteoarthritis;
platelet-rich plasma;
Xianling Gubao Capsule;
p38 mitogen-activated protein kinase
- From:
Journal of Chongqing Medical University
2025;50(5):623-629
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the therapeutic effect of platelet-rich plasma(PRP)combined with Xianling Gubao Capsule on rats with osteoarthritis(OA)and the changes in the levels of related factors.Methods:Anterior cruciate ligament transection was performed to establish a rat model of OA,and after modeling,the rats were randomly divided into control group,model group,Xianling Gubao Capsule group,PRP group,and Xianling Gubao Capsule+PRP group,with 10 rats in each group.After intervention,ELISA was used to measure the serum levels of IL-1β and TNF-α;knee articular cartilage was collected,and qPCR and Western blot were used to measure the mRNA and protein expression levels of MMP3,MMP13,Col2,Aggrecan,and p-p38;Micro-CT was used to assess the changes in bones around the knee joint;HE staining and SF staining were used to observe the changes in knee articular cartilage.Results:ELISA showed that PRP combined with Xianling Gubao Capsule significantly reduced the serum levels of the inflammatory factors such as IL-1β and TNF-α(P<0.05).The results of qPCR and Western blot showed that PRP combined with Xianling Gubao Capsule significantly reduced the expression levels of MMP3,MMP13,and p-p38 and increased the expression levels of Col2 and Ag-grecan in knee articular cartilage(P<0.05).Micro-CT,HE staining,and SF staining showed a certain degree of improvement in knee articular cartilage degeneration in the Xianling Gubao Capsule+PRP group(P<0.05).Conclusion:PRP combined with Xianling Gubao Capsule exerts a therapeutic effect on rats with OA,possibly by regulating the p38MAPK signaling pathway.
