Electroacupuncture reduces the expression of Hba-a1 and Hbb-bt and alleviates cell apoptosis after spinal cord injury by regulating the PPARγ-CD36 signaling pathway
10.13406/j.cnki.cyxb.003784
- VernacularTitle:电针调控PPARγ-CD36信号通路降低Hba-a1、Hbb-bt表达减轻脊髓损伤后细胞凋亡的研究
- Author:
Mingjiao LI
1
;
Chenglin TANG
;
Zhuxin YANG
;
Hongdi ZHAO
;
Jiapei WANG
;
Kehan XING
;
Siqin HUANG
Author Information
1. 重庆医科大学中医药学院中医防治代谢性疾病重庆市重点实验室,重庆 400016
- Keywords:
electroacupuncture;
spinal cord injury;
apoptosis;
RNA-Seq;
TMT/iTRAQ
- From:
Journal of Chongqing Medical University
2025;50(3):311-321
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a mouse model of spinal cord injury(SCI),and to investigate the effect of electroacupuncture(EA)intervention on cell apoptosis after acute SCI and its mechanism.Methods:Female C57BL/6 mice were used to establish a model of SCI,and after successful modeling,the mice were randomly divided into SCI group,EA group,and Rosiglitazone group(R group);a sham-operation group(Sham group)was also established.After successful modeling,the mice in the EA group were given EA at bilat-eral Jiaji points and Zusanli once a day for 14 days,those in the R ture,and the number of surviving cells.The EA group and the R group had a significant reduction in the expression of caspase-3 and significant increases in the expression of PPARγ and CD36,and the EA group had significant reductions in the expression of Hba-a1 and Hbb-bt.In addition,RNA-Seq and TMT/iTRAQ techniques,significant analysis,Venn analysis,and dual-omics analysis identi-fied Hba-a1 and Hbb-bt as the target genes of EA.The KEGG pathway enrichment analysis showed that EA had a significant effect on the PPAR signaling pathway.Conclusion:By regulating the PPARγ-CD36 signaling pathway,EA can promote the clearance of Hba-a1 and Hbb-bt after SCI,reduce the expression level of caspase-3,alleviate cell apoptosis,and facilitate the recovery of spinal cord nerve function.
