Preparation of polyvinyl butyral nanofiber membrane loaded with propolis and its property
10.13481/j.1671-587X.20250129
- VernacularTitle:负载蜂胶的聚乙烯醇缩丁醛纳米纤维膜的制备及其性能
- Author:
Zhihui DAI
1
;
Yungang LUO
;
Zhihui LIU
Author Information
1. 吉林大学口腔医院修复科,吉林 长春 130021
- Keywords:
Polyvinyl butyral;
Propolis;
Electrospinning;
Biocompatibility;
Antibacterial properties
- From:
Journal of Jilin University(Medicine Edition)
2025;51(1):238-244
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To prepare a polyvinyl butyral(PVB)nanofiber membrane loaded with propolis,and to clarify its physicochemical properties,drug release behavior,antibacterial activity,and biocompatibility.Methods:Propolis with a mass fraction of 0.12％was dissolved in 18％(mass fraction)PVB methanol solution.PVB and PVB-propolis(PVB-P)nanofiber membranes were prepared using electrospinning from PVB and PVB-P solutions,respectively.Scanning electron microscope(SEM)was used to observe the microscopic morphology of PVB-P nanofibers,and the Nano Measurer software was used to analyze the fiber diameter distribution.Fourier transform infrared spectroscopy(FTIR)was used to analyze the chemical composition of PVB-P.Water contact angle measurements were used to evaluate the hydrophilicity of PVB-P.UV spectrophotometer was used to detect the cumulative release of propolis at different time points.PVB and PVB-P nanofiber membranes were co-cultured with Staphylococcus aureus(S.aureus),Escherichia coli(E.coli),Candida albicans(C.albicans),Salmonella,and Pseudomonas aeruginosa(P.aeruginosa)and divided into PVB group and PVB-P group.The absorbance method was used to calculate the antibacterial values of PVB and PVB-P against the five types of bacteria.The NIH-3T3 cells were seeded on PVB and PVB-P nanofiber membranes and divided into PVB group and PVB-P group.The CCK-8 method was used to detect the survival rates of NIH-3T3 cells on the nanofiber membranes in PVB group and PVB-P group at 1,3,and 7 d.Results:The SEM results showed that PVB and PVB-P nanofibers were interconnected in a mesh-like porous structure,with uniform thickness and no beads.The Nano Measurer software measurement results showed that the diameter of PVB nanofibers was(0.50±0.10)μm,and the diameter of PVB-P nanofibers was(0.54±0.16)μm.FTIR analysis showed that PVB-P nanofiber membranes exhibited characteristic peaks of PVB(1 140 and 1 002 cm-1)and propolis(1 161 cm-1).The water contact angle measurement results showed that the contact angle of PVB membrane was(144.26°±2.90°)and that of PVB-P membrane was(128.13°±1.36°).The UV spectrophotometer results showed that the cumulative release of propolis was 0.04 mg at 1d,0.07 mg at 3 d,and reached a steady state of 0.79 mg at 7 d.The absorbance method results showed that the antibacterial values of PVB-P nanofiber membranes against S.aureus,E.coli,C.albicans,Salmonella,and P.aeruginosa were 4.39,1.27,5.68,3.16,and 1.87,respectively.The CCK-8 method results showed that the survival rates of NIH-3T3 cells on PVB and PVB-P nanofiber membranes was＞90％at 1,3,and 7 d,and there were no significant differences between various groups(P＞0.05).Conclusion:The diameter of PVB nanofiber membranes loaded with propolis is increased,and these membranes exhibit antibacterial effects against S.aureus and C.albicans,while also achieving sustained release of propolis.
