Qualitative and Quantitative Analysis of Chemical Constituents in Gualou Niubangtang by UPLC-Q-TOF-MS/MS and HPLC
10.13422/j.cnki.syfjx.20251813
- VernacularTitle:基于UPLC-Q-TOF-MS/MS和HPLC的瓜蒌牛蒡汤化学成分定性与定量分析
- Author:
Yiyi ZHANG
1
;
Jing YANG
1
;
Yuqing CHENG
1
;
Huimin GAO
1
;
Jin QIN
2
;
Li YAO
3
;
Xiyang DU
3
;
Raorao LI
1
Author Information
1. State Key Laboratory for Quality Assurance and Sustainable Use of Dao-di Herbs， Institute of Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
2. Institute of Information Traditional Chinese Medicine（TCM）， China Academy of Chinese Medical Sciences， Beijing 100700， China
3. Linyi Medical Care and Health Industry Research Institute， Linyi 276002， China
- Publication Type:Journal Article
- Keywords:
Gualou Niubangtang;
ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry （UPLC-Q-TOF-MS/MS）;
chemical constituent;
quantitative analysis;
qualitative analysis
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(6):179-187
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveThis paper aims to clarify the material basis of Gualou Niubangtang and establish a quantitative analysis method for its main constituents， providing a reference for the overall quality control of this preparation. MethodsThe constituents in the formula were systematically characterized based on ultra-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry （UPLC-Q-TOF-MS/MS）. Identification was performed by matching with the UNIFI 9.6 software and utilizing database platforms such as PubChem， ChemicalBook， and ChemSpider， combined with relevant literature reports. A quantitative analysis method for the seven main constituents in Gualou Niubangtang was established by using high performance liquid chromatography （HPLC）. ResultsUPLC-Q-TOF-MS/MS analysis identified 155 constituents， including 69 flavonoids， 36 terpenoids， 23 phenylpropanoids， 8 phenylethanoid glycosides， and 19 other types of constituents. In the established quantitative analysis method， the seven main constituents showed good linearity within their respective linear ranges. The precision， repeatability， stability， and spike recovery all met the required standards. The results showed that the content ranges of geniposide， liquiritin， hesperidin， arctiin， baicalin， oroxylin A-7-O-β-D-glucuronide， and wogonoside in 15 batches of Gualou Niubangtang were 13.67-21.25， 1.20-7.64， 5.45-7.45， 22.97-33.51， 29.95-39.07， 2.58-4.80， and 6.56-9.31 mg·g-1， respectively. ConclusionThis study successfully characterizes and attributes multi-category constituents in Gualou Niubangtang， clarifying that its material basis is primarily composed of flavonoids， terpenoids， phenylethanoid glycosides， and phenylpropanoids. Furthermore， it enables the quantification of seven constituents within the formula. This work lays a foundation for research on the quality control， action mechanism， and clinical application of this formula.
