Expression and biological role of C1GALT1 in glioblastoma
10.19405/j.cnki.issn1000-1492.2025.06.003
- Author:
Xin Ao
1
;
Yunfeng Long
1
;
Zhengrong Zhang
1
;
Mingzhu Zhang
1
;
Zhuang Le
1
;
Yanting Su
2
Author Information
1. Dept of Pharmacy , Hubei University of Science and Technology , Xianning 437100
2. Dept of Basic Medicine , Hubei University of Science and Technology , Xianning 437100
- Publication Type:Journal Article
- Keywords:
C1GALT1;
glioblastoma;
cell proliferation;
cell migration;
cell invasion;
cell senescence
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):992-999
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the expression profile of core 1 β1,3-galactosyltransferase 1(C1GALT1) in glioblastoma(GBM) and to elucidate its impact on the initiation and progression of GBM.
Methods :The expression levels and prognostic significance of C1GALT1 in GBM were analyzed using the GEPIA and CGGA databases. Two representative glioblastoma cells(U251 and LN18) were selected to construct C1GALT1-knockdown cell lines and performed in vitro experiments. The Cell Counting Kit-8(CCK-8) and Transwell assays were employed to evaluate the impact of C1GALT1 on proliferation, migration and invasion of GBM cells. Transcriptome data were analyzed to identify potential signaling pathways. Senescence β-Galactosidase Staining Kit was used to detect β-galactosidase activity.
Results :nalysis of GEPIA and CGGA databases revealed that C1GALT1 was significantly upregulated in GBM tissues compared to adjacent non-cancerous tissues (P < 0. 05) , and its high expression was associated with poor prognosis of patients (P < 0. 000 1) . The CCK-8 experiment demonstrated a significant reduction in prolifera- tion rate following C1GALT1 knockdown (P < 0. 05) . Transwell assay showed that cell migration and invasion de- creased after C1GALT1 was knocked down ( P < 0. 001) . Transcriptome sequencing and senescence β-galactosi- dase staining showed that C1GALT1 was involved in the cellular senescence signaling pathway , and the activity of β-galactosidase associated with cellular senescence significantly increased after C1GALT1 was knocked down(P < 0. 05) .
Conclusion : C1GALT1 is overexpressed in GBM tissues and may promote the proliferation , migration and invasion of GBM cells by inhibiting cellular senescence .
- Full text:2026020910482198338C1GALT1在胶质母细胞瘤中的表达和生物学作用_敖欣.pdf
