Dendrobium officinale polysaccharides ameliorate intestinal injury in neonatal mice with necrotizing enterocolitis by activating Nrf2/HO-1 pathway
10.19405/j.cnki.issn1000-1492.2025.06.002
- Author:
Jing Wang
1
;
Ming Wu
2
;
Jun Wang
2
Author Information
1. Dept of Pediatrics , Afiliated Hospital of Xuzhou Medical University , Xuzhou 221000 ; Dept of Pediatrics , The People ′s Hospital of Jiawang District of Xuzhou City , Xuzhou 221011
2. Dept of Pediatrics , Afiliated Hospital of Xuzhou Medical University , Xuzhou 221000
- Publication Type:Journal Article
- Keywords:
dendrobium;
polysaccharides;
enterocolitis;
nuclear factor erythroid 2-related factor 2;
heme oxygenase- 1;
tight junction protein;
oxidative stress
- From:
Acta Universitatis Medicinalis Anhui
2025;60(6):984-991
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To explore the effect of dendrobium officinale polysaccharides(DOP) on necrotizing enterocolitis(NEC) in neonatal mice and to preliminarily explore the potential molecular mechanisms.
Methods :Seven-day-old C57BL/6J mice were randomly divided into four groups: control(CTRL) group, necrotizing enterocolitis(NEC) group, low-dose DOP treatment(DOPL+NEC) group and high-dose DOP treatment(DOPH+NEC) group. The NEC model was established by hypoxia, cold stimulation, hypertonic feeding and intraperitoneal injection of lipopolysaccharide(LPS). At the end of the experiment, the small intestine tissues were collected. The general condition of the mice was observed, and body weight was recorded. Hematoxylin-eosin(HE) staining was used to observe pathological changes in the small intestine tissues. The expression levels of E-cadherin, Occludin and Claudin-1 were detected by immunohistochemistry and Western blot. The concentrations of tumor necrosis factor-α(TNF-α), interleukin(IL)-1β, IL-6 and IL-10 were measured by ELISA. The levels of lactate dehydrogenase(LDH), malondialdehyde(MDA), superoxide dismutase(SOD) and glutathione(GSH) were detected using commercial kits. The expression levels of nuclear factor erythroid 2-related factor 2(Nrf2) and heme oxygenase-1(HO-1) proteins were detected by immunohistochemistry and Western blot.
Results :ompared with the CTRL group , the NEC group exhibited decreased body weight and increased HE pathological scores (P < 0. 01 or P < 0. 001) . The protein expression levels of E-cadherin , Occludin and Claudin-1 were reduced (P < 0. 01 or P < 0. 001) . The concentrations of TNF-α, IL-1βand IL-6 increased , while the concentration of IL-10 decreased (P < 0. 01) . The levels of MDA and LDH increased , while the levels of GSH and SOD decreased (P < 0. 01) . The protein expression levels of Nrf2 and HO-1 increased ( P < 0. 05 or P < 0. 01) . In contrast , DOP intervention groups showed increased body weight and decreased HE scores (P < 0. 05 or P < 0. 01) . The protein expression levels of E-cadherin , Occludin and Claudin-1 increased (P < 0. 05 or P < 0. 01 or P < 0. 01) . The concentrations of TNF-α, IL-1βand IL-6 decreased(P < 0. 05 or P < 0. 01 ) , while the concentration of IL-10 increased ( P < 0. 01) . The levels of MDA and LDH decreased , while the levels of GSH and SOD increased ( P < 0. 05 or P < 0. 01) . The protein expression levels of Nrf2 and HO-1 further increased (P < 0. 05 or P < 0. 01) .
Conclusion:DOP can ameliorate the pathological damage of necrotizing enterocolitis and enhance the intestinal mucosal barrier function in NEC mice . Additionally , it can also reduce oxidative stress injury and intestinal inflammation in NEC mice . The mechanism may be associated with the activation of the Nrf2/HO-1 pathway by DOP.
- Full text:2026020910444127452铁皮石斛多糖通过激活Nrf2_HO-1通路改善新生小鼠坏死性小肠结肠炎的肠道损伤_王静.pdf
