Epidemiological surveillance and molecular profiling of brucellosis in Bozhou city, Anhui province
10.19405/j.cnki.issn1000-1492.2025.05.025
- Author:
Xiaodong Kang
1
;
Jun Wang
2
;
Shusheng Qian
3
;
Xiangying Wang
3
;
Yunfei Tang
3
;
Xuefei Huai
3
;
Dongdong Jiang
3
;
Yan Liu
4
Author Information
1. Dept of Microbiology , Anhui Medical University , Hefei 230032 ; Laboratory of Microbiology , Bozhou Center for Disease Control and Prevention , Bozhou 236800 ;
2. Laboratory of Microbiology , Anhui Center for Disease Control and Prevention , Hefei 230601
3. Laboratory of Microbiology , Bozhou Center for Disease Control and Prevention , Bozhou 236800 ;
4. Dept of Microbiology , Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
brucellosis;
pathogen surveillance;
BCSP31 _PCR;
AMOS_PCR;
16S rRNA;
brucella melitensis
- From:
Acta Universitatis Medicinalis Anhui
2025;60(5):964-970
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To illuminate the distribution of brucellosis patients and the epidemic typologies as well as the genetic attributes of brucellosis in Bozhou City,Anhui Province,thereby furnishing a substantive foundation for formulating efficacious prevention and control strategies for this disease within the region.
Methods :The rose bengal plate agglutination test(RBPT) and the tube agglutination test(TAT) were conducted on a total of 698 blood samples that had been collected.Epidemiological data of the tested subjects were meticulously collected,followed by statistical analyses of the obtained results.The genomic DNA of positive bacterial strains was cultured and extracted.Molecular identification and typing of the isolated strains were executed through 16 S rRNA sequencing.Sequence alignment was conducted employing Clustal W and MEGA 7,with comparisons made against the outcomes of AMOS-PCR and BCSP31-PCR.
Results :A total of 66 positive samples were detected through serological assays,with a positive rate of 9.46%.The demographic cohort demonstrating the highest detection rate primarily comprised individuals engaged in live sheep slaughtering.The 1 6 S rRNA gene sequencing on ten positive strains disclosed close phylogenetic affinities with Brucella melitensis.Moreover,the phylogenetic tree analysis indicated that these strains coalesced within the same branch,the findings were in alignment with the results obtained from BCSP31-PCR and AMOS-PCR assays.
Conclusion:Brucella melitensis assumes a predominant position in the transmission dynamics within this area,identifying individuals involved in sheep breeding,slaughtering,vending,and related occupations as high-risk groups.The outcomes of this study offer molecular biological substantiation for the distribution of brucellosis patients in this region,contribute to genotyping endeavors and tracing studies associated with the pathogen,and concurrently verify the efficacy of 16S rRNA molecular tracing.
- Full text:2026020516042711859安徽省亳州市布鲁氏菌病病原学监测及其分子特征分析_康晓东.pdf
