Expression of HSPA8 in breast cancer and its biological function
10.19405/j.cnki.issn1000-1492.2025.05.007
- Author:
Manman Zhang
1
;
Zheng Cheng
1
;
Jingni Zhou
2
;
Qianying Guo
2
;
Zhengsheng Wu
3
Author Information
1. ept of Pathology , School of Basic Medicine , Anhui Medical University , Hefei 230032
2. Dept of Pathology , First Afiliated Hospital of Anhui Medical University , Hefei 230022
3. ept of Pathology , School of Basic Medicine , Anhui Medical University , Hefei 230032; Dept of Pathology , First Afiliated Hospital of Anhui Medical University , Hefei 230022
- Publication Type:Journal Article
- Keywords:
HSPA8;
breast cancer;
proliferation;
invasion;
migration;
EMT
- From:
Acta Universitatis Medicinalis Anhui
2025;60(5):816-825
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of(heat shock protein a member 8,HSPA8) in breast cancer and its effect on tumor biological behaviors.
Methods:Bioinformatics analysis and immunohistochemistry assays were used to detect the expression of HSPA8 in breast cancer and adjacent non-tumor breast tissues,and the relationship between its expression and clinicopathological characteristics of breast cancer patients was analyzed.Correlation between HSPA8 expression and prognosis of breast cancer patients was analyzed by Kaplan-Meier Plotter database.HSPA8 knockdown and over expression breast cancer stabilized cells were constructed,respectively.CCK-8,clone formation,Transwell,cell scratch,Western blot and immunofluorescence assay were used to detect the effects of HSPA8 on the proliferation,invasion and migration of breast cancer cells,and its effect on epithelial-mesenchymal transition(EMT).
Results :Bioinformatics analysis and immunohistochemistry assay revealed that the expression of HSPA8 in breast cancer tissues was higher than that in adjacent non-tumour tissues(P<0.05),and its expression level of the protein was significantly and positively correlated with the tumor size,histological grade,lymph node metastasis and Ki-67 proliferation index(P<0.05).The Kaplan-Meier survival curve showed that high expression of HSPA8 was significantly associated with poor prognosis in breast cancer patients(P<0.05).CCK-8,clone formation,transwell,cell scratch,Western blot and immunofluorescence assay showed that knockdown of HSPA8 expression could significantly inhibit the proliferation,invasion,migration function and EMT of breast cancer cells(P<0.05),while overexpression of HSPA8 could significantly promote the proliferation,invasion,migration function and EMT of breast cancer cells(P<0.05).
Conclusion :HSPA8 is highly expressed in breast cancer tissues,which is closely related to disease progression and the malignant phenotype of breast cancer,suggesting that HSPA8 may be a potential biological target for breast cancer treatment.
- Full text:2026020510331652111HSPA8在乳腺癌中的表达及其生物学功能_张曼曼.pdf
