KRT14 promotes the invasion and migration of basal-like breast cancer through activating the Wnt/β-catenin pathway
10.19405/j.cnki.issn1000-1492.2025.05.006
- Author:
Zheng Cheng
1
;
Manman Zhang
1
;
Jingni Zhou
2
;
Qianying Guo
3
;
Zhengsheng Wu
3
Author Information
1. Dept of Pathology , School of Basic Medical Sciences , Anhui Medical University , Hefei 230032
2. Dept of Pathology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
3. Dept of Pathology , School of Basic Medical Sciences , Anhui Medical University , Hefei 230032; Dept of Pathology , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
- Publication Type:Journal Article
- Keywords:
KRT14;
breast cancer;
Wnt/β_catenin signaling pathway;
basal_like breast cancer;
migration;
invasion
- From:
Acta Universitatis Medicinalis Anhui
2025;60(5):805-815
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the expression of Keratin 14(KRT14) in Basal-like Breast Cancer(BLBC) and its biological functions and mechanisms.
Methods :The expression levels of KRT14 mRNA in BLBC and para-cancer breast tissues were analyzed using The Cancer Genome Atlas(TCGA) database. qPCR, Western blot(WB), and immunohistochemistry were employed to detect KRT14 expression in BLBC and adjacent normal tissues, and its correlation with clinicopathological features was analyzed. KRT14 overexpression and knockdown were performed in breast cancer cells, and cell scratch and transwell assays were performed to evaluate changes in migration and invasion abilities. To investigate the expression of proteins related to the Wnt/β-catenin signaling pathway, including catenin Beta 1(β-catenin), wingless-type MMTV integration site family, member 1(Wnt1), matrix metallopeptidase 7(MMP7), and cellular myelocytomatosis viral oncogene homolog(c-Myc), as well as the cellular localization of β-catenin, WB and immunofluorescence(IF) techniques were employed. Additionally, a Wnt/β-catenin signaling pathway inhibitor was used to verify the mechanism of action of KRT14.
Results : The expression of KRT14 was significantly higher in BLBC tissues compared to normal tissues(P<0.05), and was associated with higher T stage and histological grade(P<0.05). The overexpression of KRT14 significantly enhanced the migration and invasion abilities of breast cancer cells, while the knockdown of KRT14 significantly reduced those abilities(P<0.01). The overexpression of KRT14 can increase the expression levels of Wnt/β-catenin pathway-related proteins β-catenin, Wnt1, MMP7, and c-Myc, thereby activating the Wnt/β-catenin pathway. Moreover, the inhibition of this pathway can eliminate the effects of KRT14 on cell migration and invasion.
Conclusion:The high expression of KRT14 in BLBC may promote the migration and invasion of breast cancer cells through the Wnt/β-catenin signaling pathway.
- Full text:2026020510303799348KRT14通过激活Wnt_β-catenin通路促进基底细胞样乳腺癌侵袭和迁移_程政.pdf
