Mechanism of pachymic acid in ameliorating renal injury in pregnancy induced hypertension rats by regulating the Sirt1/PGC‑1α pathway
- VernacularTitle:茯苓酸调控Sirt1/PGC-1α通路改善妊娠高血压大鼠肾损伤的机制
- Author:
Junjiang ZHU
1
;
Jincheng LIN
1
;
Jiajian WU
2
;
Yi ZENG
3
;
Jun HU
1
;
Min LI
1
;
Hongying LIU
4
;
Jinfen LI
4
Author Information
1. Dept. of Nephrology，the Third Affiliated Hospital of Gannan Medical University，Jiangxi Ganzhou 341000，China
2. Dept. of Cardiology，the Third Affiliated Hospital of Gannan Medical University，Jiangxi Ganzhou 341000，China
3. Dept. of Gynaecology and Obstetrics，the Third Affiliated Hospital of Gannan Medical University，Jiangxi Ganzhou 341000，China
4. Dept. of Obstetrics，Ganzhou People’s Hospital，Jiangxi Ganzhou 341000，China
- Publication Type:Journal Article
- Keywords:
pachymic acid;
pregnancy induced hypertension;
renal injury;
Sirt1/PGC-1α pathway
- From:
China Pharmacy
2026;37(2):186-191
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the mechanism of pachymic acid on renal injury in pregnancy induced hypertension （PIH） rats by regulating the silent information regulator transcript 1/peroxisome proliferator-activated receptor γ coactivator-1α （Sirt1/PGC-1α） pathway. METHODS Pregnant SD rats were prepared by co-caging and PIH model was induced using N-nitro-L- arginine methyl ester （L-NAME） method. PIH rats were randomly divided into model group， L-pachymic acid （low-dose pachymic acid， 10 mg/kg） group， H-pachymic acid （high-dose pachymic acid， 20 mg/kg） group， and H-pachymic acid+EX527 （20 mg/kg pachymic acid+10 mg/kg EX527） group， with 6 rats in each group. Another 6 normal pregnant rats were selected as blank group. Each group was given relevant medicine or solvent intragastrically or intraperitoneally daily， once a day， for 28 consecutive days. After the last administration， 24 h urinary protein and tail artery systolic blood pressure （SBP） were measured in pregnant rats from each group， along with the levels of serum creatinine （Scr）， blood urea nitrogen （BUN），uric acid （UA）， and cystatin C （Cys-C）. The contents of superoxide dismutase （SOD）， malondialdehyde （MDA）， glutathione peroxidase （GSH-Px）， and 8-hydroxy-2′-deoxyguanosine （8-OHdG） in renal tissue， as well as the mRNA and protein expression levels of Sirt1 and PGC-1α， were also determined. Meanwhile， renal histopathological changes in rats from each group were evaluated using hematoxylin-eosin （HE） staining and periodic acid-Schiff （PAS） staining. RESULTS Compared with model group， L-pachymic acid group and H-pachymic acid group exhibited significant decreases in 24 h urine protein quantification， tail artery SBP， Scr， BUN， UA， Cys-C levels， glomerulosclerosis index score of renal tissue， renal tubular injury score， the percentage of PAS positive area， MDA and 8-OHdG （P＜0.05）. Conversely， the contents of SOD and GSH-Px， along with the mRNA and protein expression levels of Sirt1 and PGC-1α， were significantly increased （P＜0.05）. Moreover， these improvements were more pronounced in H-pachymic acid group （P＜0.05）. Compared with H-pachymic acid group， the aforementioned indicators in pregnant rats from the H-pachymic acid+EX527 group showed significant reversal （P＜0.05）. CONCLUSIONS Pachymic acid significantly ameliorates renal injury induced by PIH in rats， potentially through activation of the Sirt1/PGC-1α pathway.
