Identification of Chemical Constituents of Painong Powder and Constituents Absorbed into Blood by UHPLC-Q-Orbitrap-MS
10.13422/j.cnki.syfjx.20251706
- VernacularTitle:基于UHPLC-Q-Orbitrap-MS技术鉴定排脓散中化学成分和入血成分
- Author:
Han SUN
1
;
Hongsu ZHAO
2
;
Zihua XUAN
2
;
Jinwei QIAO
1
;
Fangfang ZHANG
1
;
Manqin YANG
1
;
Shuangying GUI
3
Author Information
1. The Second Affiliated Hospital of Anhui University of Chinese Medicine，Hefei 230061，China
2. Anhui Province Key Laboratory of Research & Devolopment of Chinese Medicine，Hefei 230012，China
3. School of Pharmacy，Anhui University of Chinese Medicine，Hefei 230012，China
- Publication Type:Journal Article
- Keywords:
Painong Powder;
ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry （UPLC-Q-Orbitrap-MS）;
chemical constituents;
constituents absorbed into blood;
metabolites
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(5):256-263
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo study the chemical constituents of Painong powder and the constituents absorbed into blood after oral administration to rats by ultra performance liquid chromatography-quadrupole-electrostatic field orbitrap high-resolution mass spectrometry （UPLC-Q-Orbitrap-MS）. MethodsUPLC-Q-Orbitrap-MS was employed for mass spectrometry data acquisition. The chemical constituents of Painong Powder and the constituents absorbed into blood were characterized and identified via Xcalibur 4.2 and Compound Discoverer v3.3.1 （CD） based on retention time， accurate molecular weights， secondary fragmentation ions， and comparison with reference standards and literature reports. ResultsA total of 176 chemical compounds， including 56 flavonoids， 42 triterpenoid saponins， 23 monoterpenes， 7 coumarins， 5 tannins， and other 43 compounds were identified from Painong powder. 49 components were identified in the rat plasma after oral administration of Painong powder， including 33 prototype constituents and 16 metabolites. The major metabolic pathways included hydrolysis in phase Ⅰ metabolic reactions， as well as methylation， sulfation， and glucuronidation in phase Ⅱ metabolic reaction. ConclusionThe method comprehensively identified the chemical constituents of Painong powder both in vitro and in vivo， and may provide a reference for the study of quality control and clinical applications.
