Effects of dorsal raphe 5-hydroxytryptamine neurons on sleep quality and anxiety-like behavior in PCPA insomnia model rats
10.19405/j.cnki.issn1000-1492.2025.04.016
- Author:
Quntao Li
1
;
Tongyu Du
1
;
Lei Jia
1
;
Jiangwen Yin
2
;
Yan Li
2
Author Information
1. Dept of Anesthesiology，The First Affiliated Hospital of Shihezi University，Shihezi 832000; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases，Shihezi 832000; Key Laboratory of Xinjiang Endemic and Ethnic Diseases，Shihezi 832000
2. NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, Shihezi 832000; Key Laboratory of Xinjiang Endemic and Ethnic Diseases，Shihezi 832000
- Publication Type:Journal Article
- Keywords:
5-hydroxytryptamine;
dorsal raphe;
chemical genetics;
insomnia;
anxiety;
rat
- From:
Acta Universitatis Medicinalis Anhui
2025;60(4):697-706
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the selective activation of 5-hydroxytryptamine neurons in the dorsal raphe nucleus(DRN5-HT) of rats with insomnia induced by p-chlorophenylalanine(PCPA) using chemical genetics techniques, and to explore whether it has a regulatory effect on comorbidities of insomnia and anxiety.
Methods :32 male SD rats were randomly divided into control group, PCPA model group(PCPA group), chemical activation group(hM3Dq+CNO group), and chemical control group(hM3Dq+Saline group). The anxiety level of experimental rats was evaluated through open field experiments and elevated cross maze experiments.In vivoelectroencephalography(EEG) was used to record propofol induced sleep and cortical EEG changes, and immunofluorescence staining was used to observe changes in c-Fos positive protein expression in 5-HT neurons.
Results : The behavioral results showed that compared with the chemical control group, the anxiety level of the chemical activation group was significantly lower(P<0.05), and sleep duration was significantly prolonged(P<0.05). The expression level of c-Fos positive protein in DRN5-HT neurons in the chemical activation group was higher(P<0.05). The EEG results showed that the percentage of δ-band power between the chemical activation group and the chemical control group was higher(P<0.05), the power percentage in the α-band was relatively low(P<0.05).
Conclusion :Chemical activation of 5-HT neurons in the DRN region can improve anxiety levels and increase sleep duration in PCPA induced insomnia rats.
- Full text:2026012711185344285中缝背核五羟色胺能神经元对...睡眠质量及焦虑样行为的影响_李群涛.pdf
