Investigation of the molecular difference of plasma lipids in mice with idiopathic pulmonary fibrosis based on lipidomics
10.19405/j.cnki.issn1000-1492.2025.04.008
- VernacularTitle:基于脂质组学探究特发性肺纤维化小鼠的血浆脂质分子差异
- Author:
Qingshuang Wu
1
;
Rong Qi
2
;
Chunchao Zheng
1
;
Yanan Sun
1
;
Heliang Liu
3
;
Hongli Wang
4
;
Hailan He
4
Author Information
1. School of Public Health，North China University of Science and Technology，Tangshan 063210;
2. Dept of Urology，The Ninth Division Hospital of Xinjiang Production and Construction Corps，Baiyang 834600
3. School of Public Health，North China University of Science and Technology，Tangshan 063210;; Hebei Key Laboratory of Organ Fibrosis，Tangshan 063210; Dept of Medicine， Tangshan Vocational and Technical College，Tangshan 063210
4. School of Public Health，North China University of Science and Technology，Tangshan 063210; Hebei Key Laboratory of Organ Fibrosis，Tangshan 063210
- Publication Type:Journal Article
- Keywords:
idiopathic pulmonary fibrosis;
plasma;
lipidomics;
phosphatidylcholine;
lysophosphatidylcholine
- From:
Acta Universitatis Medicinalis Anhui
2025;60(4):642-648
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the differential lipid metabolites in the plasma of mice with idiopathic pulmonary fibrosis(IPF).
Methods :Thirty SPF C57BL/6 male mice were randomly divided into 2 groups with 15 mice in each group. The experimental groups were divided into control group and bleomycin(BLM) group. The model of idiopathic pulmonary fibrosis was induced by one-time intratracheal infusion of BLM(1 mg/kg). Hematoxylin-eosin(HE) staining was used to observe the lung histopathology. The collagen fiber deposition in lung tissue was observed by Sirius red staining. The differential lipid metabolites in plasma of IPF mice were screened and enriched by lipidomics.
Results : HE staining showed that the pulmonary tissue structure was disordered, alveolar septum was broken and alveolar wall was destroyed in BLM group. Sirius red staining showed a large amount of collagen fiber deposition in the lung interstitium of BLM group. The results of lipidomics analysis showed that the lipid metabolism profile of BLM group changed, 15 differential lipid metabolites were screened out, of which 11 differential lipid metabolites were up-regulated, and 4 differential lipid metabolites were down-regulated, mainly concentrated in glycerophosphoglycerophosphates, glycerophosphocholines, steroid lactones, etc.
Conclusion :The lipid metabolism profile of BLM group mice changes, differential lipid metabolites such as phosphoglycolate phosphatase(PGP)(18:0/18:0), PGP(i-12:0/i-24:0), PGP(i-13:0/a-25:0), and phosphatidylcholine(PC)(18:0/14:0), PC(18:3/16:0), lysophosphatidylcholine(LPC)(16:1), and LPC(18:3) may play an important role in the progression of IPF. These findings provide a new reference for further study of the molecular mechanism of IPF, and also provide a potential new target for clinical treatment.
- Full text:2026012609445599994基于脂质组学探究特发性肺纤维化小鼠的血浆脂质分子差异_吴青霜.pdf
