Effects of SIRT2 regulation on migration and proliferation of cardiac fibroblasts in Ang Ⅱ-induced mice
10.19405/j.cnki.issn1000-1492.2025.04.001
- Author:
Lichan Lin
1
;
Zhiyan Liu
1
;
Zhenyu Liu
1
;
Peng Liu
1
;
Sui Sui
1
;
Yunsen Zhang
1
;
Xianwen Hu
1
;
Rui Li
1
;
Hui Tao
2
Author Information
1. Dept of Anesthesiology，The Second Hospital of Anhui Medical University，Hefei 230601
2. Dept of Anesthesiology，The Second Hospital of Anhui Medical University，Hefei 230601; Dept of Cardiothoracic Surgery， The Second Hospital of Anhui Medical University，Hefei 230601
- Publication Type:Journal Article
- Keywords:
SIRT2;
cardiac fibroblasts;
Ang Ⅱ;
proliferation;
migration;
cardiac fibrosis
- From:
Acta Universitatis Medicinalis Anhui
2025;60(4):589-595, 603
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of sirtuin 2(SIRT2) on the proliferation and migration of cardiac fibroblasts(CFs)in C57BL/6 mice under angiotensin II(Ang Ⅱ) stimulation.
Methods :The hearts were taken from 1 to 2 days C57BL/6 milk mice. After cutting and digesting, CFs were extracted by different adhesion centrifugation. After CFs attachment, the cells were cultured under control medium and Ang Ⅱ(100 nmol/L) medium and treated using OE-SIRT2 plasmid to overexpression the SIRT2 gene. RT-qPCR was used to detect mRNA expression of SIRT2 proliferating cell nuclear antigen(PCNA), periostin(POSTN)and type Ⅰ collagen procollagen A1(Col1A1), Western blot assay was used to measure the protein expression levels of SIRT2, PCNA, POSTN and Col1A1, CCK-8 assay and EdU assay were used to evaluate CFs proliferation rate, Transwell experiment was used to assess CFs migration activity.
Results:Compared with control group, Ang Ⅱ stimulation led to down-regulation of SIRT2 expression in CFs, increased collagen expression, and promoted CFs proliferation and migration. The expression of SIRT2 was up regulated in CFs treated with OE-SIRT2 plasmid under Ang Ⅱ stimulation, Col1A1, POSTN and PCNA expression was down regulated, and CFs proliferation and migration ability decreased.
Conclusion :Overexpression of SIRT2 can inhibit the proliferation and migration of CFs under Ang Ⅱ stimulation, indicating that SIRT2 may be a key regulatory point in the onset and progression of cardiac fibrosis.
- Full text:2026012609230442795SIRT2对Ang_Ⅱ诱导...维细胞增殖与迁移影响的研究_林丽婵.pdf
