A preliminary study on the effect of high-fat diet on the prognosis of post-spinal cord injury mice and its mechanism
10.19405/j.cnki.issn1000-1492.2025.03.006
- Author:
Chun Chen
1
;
Tao You
1
Author Information
1. Dept of Orthopaedics，The First Affiliated Hospital of University of Science and Technology of China，Hefei 230001; School of Clinical Medicine，School of Life Sciences and Medicine，University of Science and Technology of China，Hefei 230001
- Publication Type:Journal Article
- Keywords:
high-fat diet;
fatty acid;
palmitic acid ester;
palmitoylation modification;
spinal cord injury;
progno- sis
- From:
Acta Universitatis Medicinalis Anhui
2025;60(3):422-429
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To clarify the intrinsic link between a high-fat diet(HFD) and the pathological progression and prognosis of spinal cord injury(SCI) while preliminarily exploring the potential underlying mechanisms.
Methods:SCI models were established in mice that were fed either a regular diet(RD) or HFD, with injury inflicted specifically on the T9-T12segments. Hematoxylin-Eosin(HE) staining, Masson staining, and Nissl staining were used to observe the local histological changes in SCI tissues. The basso, beattie, and bresnahan(BBB) score and footprint analysis were used to evaluate and compare hindlimb functional recovery after SCI in both RD and HFD mice.In vitroexperiments were conducted to identify key fatty acids in the HFD that exacerbate neuronal damage, whilein vivoexperiments assessed the effects of 2-bromopalmitate(2-BP), a palmitic acid inhibitor, on HFD-fed mice with SCI.
Results :Compared to RD-fed mice, HFD-fed mice exhibited significantly larger lesion areas, more severe neuronal damage, and poorer hindlimb functional recovery after SCI. Palmitic acid was identified as the key fatty acid aggravating neuronal damage. Further more, inhibition of palmitoylation, mediated by palmitic acid, enhanced neuronal survival, promoted tissue repair, and improved hindlimb functional recovery in HFD-fed mice post-SCI.
Conclusion:HFD exacerbates pathological damage following SCI in mice through palmitic acid, impairing recovery. Palmitic acid-mediated palmitoylation is likely the main mechanism underlying this effect.
- Full text:2026012217163054960高脂膳食对小鼠脊髓损伤预后的影响及其机制的初步研究_陈淳.pdf
