Expression and functional mechanism of PPFIA4 gene in hepatocellular
10.19405/j.cnki.issn1000-1492.2025.03.005
- Author:
Haodong Cui
1
;
Jimin Yin
1
;
Kai Guo
2
;
Bijun Wang
3
;
Tianbing Wang
2
;
Wenyong Wu
1
Author Information
1. Dept of General Surgery，Anhui No．2 Provincial People 's Hospital Clinical College of Anhui Medical University，Hefei 230041; The Fifth Clinical Medical College of Anhui Medical University，Hefei 230032; Anhui Provincial Key Laboratory of Occupational Health，Anhui No．2 Provincial People 's Hospital，Hefei 230041; Dept of General Surgery，Anhui No．2 Provincial People 's Hospital，Hefei 230041;
2. Dept of General Surgery，Anhui No．2 Provincial People 's Hospital，Hefei 230041
3. Dept of Surgery，Clinical College of Anhui Medical University，Hefei 230012
- Publication Type:Journal Article
- Keywords:
hepatocellular carcinoma;
PPFIA4;
prognosis;
migration;
invasion;
EMT
- From:
Acta Universitatis Medicinalis Anhui
2025;60(3):414-421
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression level of PPFIA4 in hepatocellular carcinoma tissues and HCCLM3 cells and its regulation of the biological behavior of hepatocellular carcinoma.
Methods :Bioinformatics analysis, Western blot, and immunohistochemistry were employed to detect the expression of PPFIA4 in tumor tissues of patients with hepatocellular carcinoma and analyze the prognosis of these patients. An siRNA plasmid was designed to knock down the expression of PPFIA4 in HCCLM3 cells. The effects of PPFIA4 knockdown on the migration and invasion abilities of HCCLM3 cells were then evaluated using scratch healing and Transwell assays. Furthermore, Western blot was utilized to detect the expression levels of epithelial-mesenchymal transition(EMT)-related protein markers in the HCCLM3 cell line after transfection with the siRNA plasmid.
Results:PPFIA4 was highly expressed in hepatocellular carcinoma tissues and hepatocellular carcinoma cells( HCCLM3, Li-7, MHCC97H); the high expression of PPFIA4 indicated that the clinical stage of patients was late and the overall survival(OS) was short. After knocking down the expression of PPFIA4 in HCCLM3 cell line, the migration and invasion ability of HCCLM3 cells decreased(P<0.001) and the expression of EMT markers changed. The expression of epithelial cell marker E-cadherin increased(P<0.01), while the expression of mesenchymal markers Vimentin and N-cadherin decreased(P<0.05,P<0.01).
Conclusion:PPFIA4 is highly expressed in hepatocellular carcinoma tissues and hepatocellular carcinoma cell lines and is associated with poor prognosis of patients. Silencing PPFIA4 can regulate the biological behavior of hepatocellular carcinoma cells and inhibit the migration and invasion of HCCLM3 cells. The specific mechanism may be related to EMT.
- Full text:2026012217140259975PPFIA4基因在肝细胞癌中的表达及作用机制_崔浩东.pdf
