Cinnamaldehyde inhibits growth, metastasis and induces apoptosis of human endometriotic cells through RPS7
10.19405/j.cnki.issn1000-1492.2025.03.004
- Author:
Xiaoxuan Zhan
1
;
Chengyi Liu
1
;
Jiahua Peng
1
;
Shuzhen Liu
1
;
Xin Li
1
;
Yunying Ren
1
;
Danni Chen
1
;
Peishuang Li
2
;
Ruining Liang
3
Author Information
1. Jiangxi University of Traditional Chinese Medicine Graduate School，Nanchang 330004;
2. Dept of Gynecology，Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine， Nanchang 333006
3. Dept of Gynecology，Second Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine，Nanchang 330012
- Publication Type:Journal Article
- Keywords:
cinnamaldehyde;
RPS7;
endometriosis;
proliferation;
migration;
invasion;
apoptosis
- From:
Acta Universitatis Medicinalis Anhui
2025;60(3):405-413
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effects of cinnamaldehyde(CA) on the growth, metastasis and apoptosis of human endometriosis(EMs) cells and to explore whether the mechanism is related to ribosomal protein S7(RPS7) expression.
Methods : Endometriosis cells were divided into control group, CA group, sh-NC group, CA+sh-RPS7 group. Effects of CA on cell growth in human endometriotic cells were determined using Cell Counting Kit-8(CCK-8) and colony formation assay. Effects of CA on cell metastasis were performed by motility assay and Transwell assay. Effects of CA on cell apoptosis were evaluated by Hoechst 33258 staining and flow cytometry. Meanwhile, the levels of PCNA, E-cadherin, Vimentin, Bax and Bcl-2 were evaluated using Western blot in human endometriotic cells with treatment CA. The expression of RPS7 was detected by qRT-PCR and Western blot assay. The RPS7 overexpression of human endometriotic cells was established by cell transfection. CA-mediated effects on cell proliferation and apoptosis were determined by CCK-8 assay and flow cytometry in human endometriotic cells with RPS7 overexpression.
Results :CA repressed cell growth as well as down-regulated PCNA. The half inhibitory concentration(IC50) value was 53.60 μmol/L after 24 h treatment, and colony formation rate was 25.32%. Additionally, CA inhibited metastasis which was associated with downregulated Vimentin and upregulated E-cadherin. The relative migration rates were 35% and 29% as well as invasion rate was 40%. Further, CA induced apoptosis by cell cycle G2/M phase arrest and cell apoptosis rate was 25.1%, which related to the up-regulation of of Bax and the down-regulation of Bcl-2. CA inhibited the expression of RPS7 and overexpression of RPS7 promoted cell proliferation and suppressed apoptosis in CA-mediated cells.
Conclusion : CA inhibits cell growth, metastasis, and induces cell apoptosis by downregulating the expression of RPS7.
- Full text:202601221710135439桂皮醛调控RPS7表达抑制...症细胞生长、转移及诱导凋亡_詹晓璇.pdf
