Effects of laminarin on non-proliferative diabetic retinopathy in C57BL/6 mice based on transcriptomics analysis
10.19405/j.cnki.issn1000-1492.2025.03.002
- Author:
Lei Zhang
1
;
Sumei Zhang
2
;
Zhen Yang
2
;
Weikang Hu
2
;
Hongmei Bai
2
;
Wenjing Zhou
2
;
Zihan Wang
2
;
Mingcong Li
3
;
Shengquan Zhang
2
;
Rongfeng Liao
4
Author Information
1. Dept of Ophthalmology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022; Dept of Ophthalmology，Fuyang People 's Hospital of Anhui Medical University，Fuyang 236012
2. Dept of Biochemistry and Molecular Biology，School of Basic Medical Sciences，Anhui Medical University，Hefei 230032
3. Dept of Pathology，Anhui Medical University Affiliated Hefei Hospital ( The Second People 's Hospital of Hefei) ，Hefei 230022
4. Dept of Ophthalmology，The First Affiliated Hospital of Anhui Medical University，Hefei 230022
- Publication Type:Journal Article
- Keywords:
high throughput sequencing;
laminarin;
diabetes retinopathy;
C57BL /6
- From:
Acta Universitatis Medicinalis Anhui
2025;60(3):392-398
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of laminarin(LAM) on nonproliferative diabetes retinopathy by high throughput sequencing(RNA-seq).
Methods :The diabetes model was established by intraperitoneal injection of streptozotocin(STZ), and the effect of LAM on diabetic mice was observed.C57BL/6 mice were randomly divided into three groups: Control group, Model group, and LAM group, with 8 mice in each group. After 8 weeks of modeling, the LAM group received a 4-week intraperitoneal injection of LAM treatment. Changes in blood glucose and body weight of the three groups of mice were recorded, HE staining was performed to examine retinal lesions, and RNA-seq was used to identify differentially expressed genes(DEGs) in diabetic retinopathy(DR) under the action of STZ and LAM.
Results :STZ successfully established the model of DR, and LAM reduced the blood sugar in diabetic mice to a certain extent and improved the pathological morphology of retinal structural looseness in diabetic mice. After RNA-seq analysis of DEGs, it was found that there were a total of 214 DEGs in the retina of the Model group mice compared to the Control group. Enrichment analysis revealed that DR could exacerbate the lesions through the PI3K Akt signaling pathway. There were a total of 42 DEGs in the retina of the Model group and LAM group mice, and enrichment showed that LAM improved the lesions through the neutrophil extracellular trap pathway. Early growth response factor 1(Egr1), FBJ osteosarcoma oncogene(Fos), nuclear receptor subfamily 4A member 1(Nr4a1), and salt-induced kinase 1(Sik1) were regulated by STZ, and LAM significantly regulated their expression, which might be closely related to LAM′s treatment of diabetic retinopathy.
Conclusion:DEGs can exacerbate the severity of diabetic retinopathyviathe PI3K-Akt signaling pathway. LAM can mitigate diabetic retinopathyviathe neutrophil extracellular trap pathway. Egr1, Fos, Nr4a1, and Sik1 are key genes involved in LAM treatment of STZ-induced DR.
- Full text:2026012217010838536基于转录组学分析昆布多糖对...生性糖尿病视网膜病变的影响_张蕾.pdf
