Sinapine alleviates lung tissue inflammation and mucus hypersecretion in asthmatic mice by inhibiting Notch2/Notch3-Hes1 signal pathway
10.19405/j.cnki.issn1000-1492.2025.02.014
- Author:
Hongmei Tang
1
;
Xiaoyun Wang
1
;
Jian Wang
2
;
Yun Zhang
3
;
Zhibin Wang
1
;
Xiefang Yuan
1
;
Xing Wang
1
;
Guofeng Xu
1
;
Gang Qin
4
;
Yuejiao Li
1
Author Information
1. nflammation and Allergic Diseases Research Unit , The Afiliated Hospital of Southwest Medical University , Luzhou 646000
2. Health Management Center , The Afiliated Hospital of Southwest Medical University , Luzhou 646000
3. nflammation and Allergic Diseases Research Unit , The Afiliated Hospital of Southwest Medical University , Luzhou 646000; Dept of Respiratory and Critical Care Medicine , The Afiliated Hospital of Southwest Medical University , Luzhou 646000
4. Dept of Otolaryngology-Head and Neck Surgery , The Afiliated Hospital of Southwest Medical University , Luzhou 646000
- Publication Type:Journal Article
- Keywords:
sinapine;
asthma;
Notch pathway;
proinflammatory cytokine;
Muc5ac;
airway inflammation;
mucus hypersecretion
- From:
Acta Universitatis Medicinalis Anhui
2025;60(2):286-292
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the effects of sinapine on lung tissue inflammation and mucus hypersecretion in asthmatic mice.
Methods:Eight-week-old female C57BL/6J mice were randomly divided into Control group, ovalbumin(OVA) group, Sinapine group, and Sinapine+OVA group. The asthmatic mice model were established by intraperitoneal injection of OVA combined with aluminum hydroxide [Al(OH)3] suspension and OVA nasal stimulation. One hour before OVA nasal stimulation, the mice in Sinapine+OVA group and Sinapine group were intraperitoneally injected with sinapine solution, and the mice in OVA group and Control group were treated with the same dose of 0.9% sodium chloride solution. 24 hours after the last OVA stimulation, the inflammation of lung tissue of mice were observed by HE staining; the mucus secretion were evaluated by PAS staining; the mRNA expression levels of Interleukin-4(IL-4), Interleukin-5(IL-5), Interleukin-13(IL-13), tumor necrosis factor-alpha(TNF-α), Mucin 5ac(Muc5ac), and the mRNA of the key genes of Notch pathway such as Notch receptor 1(Notch1), Notch receptor 2(Notch2), Notch receptor 3(Notch3), and hes family bHLH transcription factor 1(Hes1) in lung tissues were detected by real-time fluorescent quantitative PCR(RT-qPCR); the expression levels of Notch1, Notch2, Notch3 and Hes1 proteins were determined by Western blot.
Results : Compared with Control group, the inflammation score and PAS score of lung tissues of mice in OVA group increased(P<0.001); the mRNA expression levels of IL-4, IL-5, IL-13, TNF-α, and Muc5ac of mice in OVA group were enhanced(P<0.05); the mRNA and protein expression levels of Notch2, Notch3, and Hes1 of mice in OVA group significantly increased(P<0.001), while there was no significant difference in the mRNA and protein expression levels of Notch1. Compared with OVA group, the inflammation score and PAS score of lung tissues of mice in Sinapine+OVA group decreased(P<0.001); the mRNA expression levels of IL-4, IL-5, IL-13, TNF-α, and Muc5ac of mice in Sinapine+OVA group were reduced(P<0.05); the mRNA and protein expression levels of Notch2, Notch3, and Hes1 of mice in Sinapine+OVA group were downregulated(P<0.05), while there was no significant difference in the mRNA and protein expression levels of Notch1.
Conclusion :Sinapine can alleviate the lung tissue inflammation and mucus hypersecretion in asthmatic mice, and its mechanism may be related to the inhibition of Notch2/Notch3-Hes1 signal pathway.
- Full text:202601221105589661芥子碱通过抑制Notch2...小鼠肺组织炎症和黏液高分泌_唐红梅.pdf
