Effect of WTAP on collagen deposition in bleomycin-induced pulmonary fibrosis
10.19405/j.cnki.issn1000-1492.2025.02.011
- Author:
Yunsen Yunsen
1
;
Zhenyu Liu
1
;
Zhiyan Liu
1
;
Lichan Lin
1
;
Jiming Sha
2
;
Hui Tao
1
;
Qi Chen
1
Author Information
1. Dept of Anesthesiology and Perioperative Medicine , The Second Afiliated Hospital of Anhui Medical University, Hefei 230601; Key Laboratory of Anesthesiology and Perioperative Medicine of Anhui Higher Education Institutes , Hefei 230601
2. Dept of Thoracic Surgery, The Second Afiliated Hospital of Anhui Medical University, Hefei 230601
- Publication Type:Journal Article
- Keywords:
Wilms/ tumor 1-associated protein;
pulmonary fibrosis;
bleomycin;
epitranscriptome;
collagen;
N6- methyladenosine
- From:
Acta Universitatis Medicinalis Anhui
2025;60(2):266-271
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the effect of Wilms′ tumor 1-associated protein(WTAP) on tissue collagen deposition in pulmonary fibrosis caused by bleomycin.
Methods :60 mice were randomly divided into four groups: control group(Control group), Bleomycin-induced pulmonary fibrosis group(BLM group), pulmonary fibrosis lentivirus empty vector control group(BLM+LV-NC group), pulmonary fibrosis WTAP lentivirus group virus group(BLM+LV-WTAP group). Experimental pulmonary fibrosis mouse model was established by subcutaneous injection of bleomycin(35 mg/kg) into the abdomen, twice a week for a total of 8 times. After modeling, Western Blot was used to detect the protein expression of fibrosis-related markers α-smooth muscle actin(α-SMA), type I collagen(Collagen Ⅰ), fibronectin(Fibronectin), and WTAP protein. Masson staining and Sirius Red staining were used to detect collagen deposition. RT-qPCR was used to detect WTAP mRNA expression, WTAP lentivirus infection effect, and Collagen Ⅰ mRNA expression.
Results: Compared with the Control group, the expression of pulmonary fibrosis markers α-SMA(P<0.001), Collagen Ⅰ(P<0.001), and Fibronectin(P<0.01) protein in the BLM group all increased. Masson staining(P<0.001) and Sirius Red staining(P<0.001) confirmed that significant collagen deposition occurred in the lung tissue of the BLM group. In addition, the expression of WTAP protein in the lung tissue of the BLM group increased(P<0.01). Compared with the Control group, the expression of WTAP mRNA in the BLM group increased(P<0.001). Compared with the BLM+LV-NC group, the expression of WTAP mRNA in the tissues of the BLM+LV-WTAP group decreased(P<0.001), proving that virus infection is effective. After infection with WTAP lentivirus, collagen fiber deposition decreased(P<0.001), Collagen Ⅰ mRNA(P<0.001) level decreased, and protein(P<0.001) expression decreased in the BLM+LV-WTAP group.
Conclusion :Knocking down of WTAP can reduce collagen deposition in bleomycin-induced pulmonary fibrosis tissue in mice and improve experimental pulmonary fibrosis.
- Full text:2026012210531018143WTAP对博来霉素诱导的肺纤维化胶原沉积的影响_张云森.pdf
