Whole genome sequencing of Bifidobacterium animalis subsp. lactic B4 and analysis of its probiotic properties
10.19405/j.cnki.issn1000-1492.2025.02.010
- Author:
Ruipei Ding
1
;
Chengzhong Liu
2
;
Cancan Shi
2
;
Huiyu Zhang
1
;
Yiping Dai
2
;
Ruxue Mu
2
;
Zilong Li
2
;
Maozhang He
2
;
Zhuojun Li
3
Author Information
1. The First Clinical School of Medicine , Anhui Medical University, Hefei 230032
2. Dept of Microbiology, Anhui Medical University, Hefei 230032
3. Dept of Basic Medicine , Fuzhou Medical College of Nanchang University, Fuzhou 344000
- Publication Type:Journal Article
- Keywords:
Bifidobacterium animalis;
isolation and culture;
whole genome sequencing;
genome analysis;
probiotics;
probiotic gene
- From:
Acta Universitatis Medicinalis Anhui
2025;60(2):255-265
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate whole genome information of a newly isolatedBifidobacterium animalissubsp. lactic B4 strain from healthy human feces was analyzed and its probiotic properties.
Methods :The antimicrobial resistance, hemolytic, gastric acid tolerance and biochemical characteristics of B. animalis B4 were evaluated byin vitroexperiments, and its whole genome was sequentially sequenced and functional annotation was performed by next and three-generation sequencing technology.
Results:Whole genome sequencing of B. animalis B4 showed that its genome size was 1 944 146 bp, with GC content of 60.49%, no plasmid, and a total of 1 642 genes. The results ofin vitroanalysis showed that the B. animalis B4 had good probiotic properties, including non-hemolytic and stomach acid resistance. At the same time, the genome results showed that the B. animalis B4 strain did not have toxin and disease-related genes, drug resistance genes were few and the transmission ability was not high, so it had high safety. Gene annotation of KEGG, COG and GO showed that it contained many biological active enzymes, such as β-galactosidase, L-lactate dehydrogenase and other probiotic genes.
Conclusion: The B. animalis B4 has good probiotic properties, showing excellent safety at the genetic level, with a probiotic gene sequence.
- Full text:2026012210451241583动物双歧杆菌B4株的全基因组测序及其益生特性分析_丁瑞培.pdf
