The mechanism of CD151 regulating vascular permeability through vesicle internalization and recycling
10.19405/j.cnki.issn1000-1492.2025.02.005
- Author:
Shilang Fan
1
;
Luying Jiang
2
;
Zixuan Zhang
3
;
Mengmeng Ji
3
;
Houjuan Zuo
4
;
Jingbo Liu
5
Author Information
1. Dept of Cardiology , Tianyou Hospital Afiliated to Wuhan University of Science and Technology , Wuhan 430064;
2. Dept of Child Health , Wuhan Children ′s Hospital (Wuhan Maternal and Child Health Hospital) , Tongji Medical College , Huazhong University of Science and Technology , Wuhan 430016; Tongji Medical College of HUST , Wuhan 430014; Dept of Cardiology, Tongji Hospital Tongji Medical College of HUST , Wuhan 430014
3. Tongji Medical College of HUST , Wuhan 430014; Dept of Cardiology, Tongji Hospital Tongji Medical College of HUST , Wuhan 430014
4. Dept of Cardiology , Tianyou Hospital Afiliated to Wuhan University of Science and Technology , Wuhan 430064; Tongji Medical College of HUST , Wuhan 430014; Dept of Cardiology, Tongji Hospital Tongji Medical College of HUST , Wuhan 430014
5. Dept of Child Health , Wuhan Children ′s Hospital (Wuhan Maternal and Child Health Hospital) , Tongji Medical College , Huazhong University of Science and Technology , Wuhan 430016
- Publication Type:Journal Article
- Keywords:
acute lung injury;
CD151;
internalized recycling;
endothelial cells;
vascular permeability;
VE-cad- herin
- From:
Acta Universitatis Medicinalis Anhui
2025;60(2):218-225, 233
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To explore the effect and mechanism of CD151 on vascular permeability by regulating vesicle internalization and recycling.
Methods:Wild-type mice and CD151 knockout mice were divided into WT-con group, WT-model group, KO-con group and KO-model group, with 6 mice in each group. WT-model group and KO-model group were intraperitoneally injected with LPS to prepare sepsis ALI model, and WT-con group and KO-con group were intraperitoneally injected with phosphate buffer saline(PBS) as a control. 24 h after modeling, pulmonary vascular permeability was measured by Miles test. The siRNA silencing CD151 expression(si-CD151) and negative control si-NC were transfected into EA.hy 926 cells. The permeability of endothelial cell layer to FITC-dextran at different time points was observed under basic conditions and vascular endothelial growth factor-A(VEGF-A) stimulation conditions. Transcriptome sequencing of endothelial cells in si-CD151 group and si-NC group; the distribution and internalization of CD151 in each group were measured using immunofluorescence. Western blot and real-time quantitative RT-qPCR were used to detect the expression of VE-cadherin in si-CD151 groupand other groups. The distribution and internalization of VE-cadherin in each group were measured using immunofluorescence.
Results :Miles experiment results indicated that dye exudation in lung tissue of WT-model group was significantly higher than that of WT-con group(P<0.01). The dye exudation in the lung tissue of KO-model group increased compared with WT-model group(P<0.05). The results of endothelial cell layer permeability test showed that the permeability of FITC-dextran in si-CD151 group was significantly higher than that in control group after VEGF-A stimulation for 30, 60 and 120 min(P<0.05). Transcriptome sequencing results suggested that CD151 in endothelial cells was closely related to vesicle-mediated transport. Compared with other groups, protein and mRNA levels of VE-cadherin in CD151 knockdown endothelial cells was significantly lower(allP<0.01). The immunofluorescence assay demonstrated that after VEGF-A stimulation, the decrease of CD151 expression significantly impaired the expression of VE-cadherin at cell-cell contacts and reduced the CD151-VE-cadherin colocalization in the perinuclear region compared with other groups.
Conclusion:The absence of CD151 affects the internalization and recycling of endothelial cell vesicles, affects the expression and internalization of VE-cadherin, and then influences vascular permeability.
- Full text:2026012210221628952CD151通过囊泡内化再循环调控血管通透性的机制_范诗浪.pdf
