Preparation of allitridin nano-sustained release particles and experimental study on its protective effect on lower limb ischemia
10.19405/j.cnki.issn1000-1492.2025.02.003
- Author:
Huan Ouyang
1
;
Bo Liu
2
;
Yi Liu
2
;
Binshan Zha
1
;
Yang Ding
1
;
Xianyu Hu
1
;
Zhiyong Chen
1
Author Information
1. Dept of General Surgery , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
2. Dept of General Surgery , The First Afiliated Hospital of Anhui Medical University , Hefei 230022 ; The First Clinical College of Anhui Medical University , Hefei 230032
- Publication Type:Journal Article
- Keywords:
allitridin;
aiallyl trisulfide;
hollow mesoporous silicon nanoparticles;
lower limb ischemia;
hydrogen sulfide;
nano-drug delivery system
- From:
Acta Universitatis Medicinalis Anhui
2025;60(2):201-209
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To prepare hollow mesoporous silicon nanoparticles ( HMSNs) loaded with allicin—diallyl trisulfide (DATS) , and to study their feasibility as a therapeutic agent for ischemic injury of lower limbs .
Methods:HMSNs were synthesized by selective etching , and their microstructure was observed by scanning and transmis- sion electron microscopy. Their physical and chemical properties were analyzed by X-ray diffraction and dynamic light scattering (DLS) . Their biological safety was tested by erythrocyte hemolysis and cytotoxicity experiments . DATS was loaded into HMSNs by adsorption to obtain DATS sustained release nanoparticles (DATS-HMSNs) , and the cumulative release curve of DATS was calculated and produced by ultraviolet spectrophotometry. C57BL/6 mice were randomly divided into four groups (sham operation group , normal saline group , DATS group , and DATS-HM- SNs group) . Lower limb ischemia models were made by femoral artery ligation and resection . The exercise ability and the contents of tumor necrosis factor alpha (TNF-α ) , interleukin-6 (IL-6) , monocyte chemoattractant protein- 1 (MCP-1) , reactive oxygen species (ROS) , platelet-endothelial cell adhesion molecule (CD31) , alpha smooth muscle actin ( α-SMA) , basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in muscles of mice in each group before and after limb ischemia were tested .
Results :Scanning and transmission e- lectron microscope observation showed that the prepared HMSNs were hollow , spherical and uniform in particle size . DLS results showed that the particle size was (226. 5 ± 11 . 8) nm. The results of red blood cell hemolysis test and cytotoxicity test showed that HMSNs had good biocompatibility. The maximum drug loading rate of HMSNs on DATS was 27. 89% , the cumulative release rate of DATS in 7 days was about 80. 12% , and could reach 97. 27% in 21 days . Compared with the control group , after DATS-HMSNs were applied to mice with lower limb ischemia , immunohistochemical staining showed that the levels of CD31 , α-SMA , bFGF and VEGF increased ( P < 0. 05) . Elisa test showed that the levels of TNF-α , IL-6 , MCP-1 and ROS decreased (P < 0. 05) , and the exercise ability of mice recovered satisfactorily after ischemia.
Conclusion :DATS-HMSNs can release DATS slowly and continu- ously , providing protection against ischemic injury of lower limbs .
- Full text:2026012209242317738大蒜新素纳米缓释颗粒的制备...下肢缺血保护作用的实验研究_欧阳欢.pdf
