Study on the antagonistic activity of Lactococcus garvieae SHAMU-LG6 against Staphylococcus
10.19405/j.cnki.issn1000-1492.2025.02.002
- Author:
Shengnan Weng
1
;
Guiyun Leng
1
;
Ying Liu
2
;
Yawu Wang
1
;
Xin Li
1
;
Jie Yao
1
;
Qiang Zhou
1
;
Wei Tang
1
Author Information
1. Dept of Clinical Laboratory , The Second Afiliated Hospital of Anhui Medical University , Hefei 230601
2. High Magnetic Field Laboratory, Hefei Institutes of Physical Science , Chinese Academy of Sciences , Hefei 230031
- Publication Type:Journal Article
- Keywords:
Lactococcus garvieae;
Staphylococcus;
antagonistic activity;
antimicrobial active components;
separa- tion and purification;
16S rDNA;
oxford cup method
- From:
Acta Universitatis Medicinalis Anhui
2025;60(2):195-200
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the antagonistic activity of Lactococcus garvieae SHAMU-LG6 against Staphy- lococcus .
Methods :VITEK 2 GP identification card , Microflex LT MALDI-TOF mass spectrometer and 16S rDNA amplification sequencing were used to identify the strain species . The antagonistic activity of L. garvieae SHAMU- LG6 against different Staphylococcus was detected by Oxford cup method for bacterial inhibition ; the antimicrobial active components were preliminarily isolated and purified by adsorption on XAD16 nonionic macroporous resin , gradient ethanol elution and rotary evaporation drying.
Results :L. garvieae SHAMU-LG6 exhibited potent antago- nistic effect against methicillin-resistant Staphylococcus aureus , methicillin-susceptible S. aureus , S. epidermidis , S. saprophyticus , S. lugdunensis , S. hominis , S. capitis and S. warneri , with inhibitory indices of 3 . 3 , 3 . 0 , 4. 3 , 2. 0 , 4. 0 , 3 . 5 , 3 . 8 , and 3 . 5 , respectively. The antimicrobial active components produced by L. garvieae SHAMU-LG6 were mainly present in 70% and 80% ethanol eluates .
Conclusion : L. garvieae SHAMU-LG6 ex- hibits a potent antagonistic effect on Staphylococcus , and the antimicrobial active components produced by it are ex- pected to be a lead compound for the development of novel antimicrobial agents .
- Full text:2026012209200630730格氏乳球菌SHAMU-LG6对葡萄球菌的拮抗活性研究_翁胜男.pdf
