Study on the mechanism of long non-coding RNA LUCAT1 regulating the malignancy of pancreatic cancer MIA PaCa-2 cells
10.19405/j.cnki.issn1000-1492.2025.02.001
- Author:
Mengjie Ye
1
;
Weiwei Qu
1
;
Guangtao Luo
1
Author Information
1. Dept of Breast Surgery , The First Afiliated Hospital of Anhui Medical University , Hefei 230022
- Publication Type:Journal Article
- Keywords:
long non-coding RNA;
LUCAT1;
pancreatic cancer;
MIA PaCa-2;
tumor drug resistance;
Akt
- From:
Acta Universitatis Medicinalis Anhui
2025;60(2):187-194
- CountryChina
- Language:Chinese
-
Abstract:
Objective :To investigate the effect of long non-coding RNA LUCAT1 (lncRNA LUCAT1) on the biological behavior of MIA PaCa-2 in human pancreatic cancer cells , and to explore the potential role of LUCAT1 in the malignant progression of pancreatic cancer.
Methods :The mutation and expression of LUCAT1 in pancreatic cancer were analyzed by GEPIA , the expression levels of LUCAT1 in human pancreatic ductal cells HPNE and hu- man pancreatic cancer cells were detected by q-PCR , and the expression and distribution of LUCAT1 in human pancreatic cancer tissues were detected by FISH . CCK-8 assay and Transwell assay were used to detect the effects of LUCAT1 on the proliferation , apoptosis , drug resistance , and migration of MIA PaCa-2 cells . Gene ensemble enrichment analysis was performed to compare the related signaling pathways involved in LUCAT1 , and Western blot assay was used to verify the protein expression level .
Results :The results of GEPIA analysis showed that the expression level of LUCAT1 in human pancreatic cancer tissues was up-regulated , and the expression of LUCAT1 in human pancreatic cancer cells was significantly higher ( P < 0. 05 ) . Knockdown and overexpression of LUCAT1 could affect the proliferation , apoptosis , gemcitabine resistance , migration and invasion of pancreatic cancer cells , and the differences were statistically significant (P < 0. 05) . In addition , LUCAT1 affected p-Akt expression levels in pancreatic cancer and was inhibited after treatment with Akt inhibitor MK-2206 .
Conclusion :LUCAT1 regulates the malignant progression of MIA PaCa-2 in pancreatic cancer cells through the PI3K-Akt signaling pathway.
- Full text:202601220915585010长链非编码RNA_LUCA...aCa-2细胞恶性机制研究_叶梦洁 (1).pdf
