Progress of cross-linking mass spectrometry based on liquid chromatography-mass spectrometry technology in the research of protein-protein interactions
10.11665/j.issn.1000-5048.2025021401
- VernacularTitle:基于液质联用技术的交联质谱用于蛋白质相互作用研究的进展
- Author:
Maolin WANG
1
;
Yan LIU
;
Fang YAN
;
Guibin SUN
Author Information
1. 中国药科大学药物分析系, 南京 210009;哈尔滨工业大学医院药局, 哈尔滨 150000;南京希罗斯医药有限公司, 南京 210038
- Publication Type:Journal Article 期刊文章
- Keywords:
cross-linking mass spectrometry;
protein-protein interactions;
protein structure;
proteomics
- From:
Journal of China Pharmaceutical University
2025;56(6):793-802
- CountryChina
- Language:Chinese
-
Abstract:
Cross-linking mass spectrometry (XL-MS) technology has become a key tool for identifying protein-protein interactions (PPIs) and revealing the protein structures in living cells, tissues, or organelles. The XL-MS technology has demonstrated remarkable superiority compared with conventional methods for studying PPIs and protein structures. In the past few years, new cross-linker structures have been arising continuously. They have not only broadened the range of cross-linking reactions but also provided additional methods for the enrichment of cross-linking peptides. Moreover, owing to the advancement of computer software and artificial intelligence, cross - linking data search engines and visualization software have also been enhancing the speed and accuracy of cross-linking peptide identification. The evolution of cross-linkers and cross-linking peptide search engines has promoted the application of the XL-MS technology in the research on protein-protein interactions (PPIs) within organelles and even living cells and in the analysis of protein structures.
