Exploration of the mechanism of action of Sizi Dingchuan granules in the treatment of bronchial asthma based on UHPLC-Q-Orbitrap HRMS technology and network pharmacology
10.11665/j.issn.1000-5048.2025021202
- VernacularTitle:基于UHPLC-Q-Orbitrap HRMS技术和网络药理学探讨四子定喘颗粒治疗支气管哮喘的作用机制
- Author:
Ying ZHANG
1
;
Wumaier Gulisire
;
Chenyu YE
;
De JIANG
;
Haiying ZHANG
Author Information
1. 新疆医科大学第四临床医学院, 乌鲁木齐 830000;新疆医科大学中医学院, 乌鲁木齐 830000;新疆医科大学附属中医医院, 乌鲁木齐 830000;新疆中药炮制研究重点实验室, 乌鲁木齐 830000
- Publication Type:Journal Article 期刊文章
- Keywords:
Sizi Dingchuan granules;
bronchial asthma;
ultrahigh-performance liquid chromatograph-hybrid quadrupole orbitrap high resolution mass spectrometry;
cleavage pathway;
network pharmacology
- From:
Journal of China Pharmaceutical University
2025;56(6):737-746
- CountryChina
- Language:Chinese
-
Abstract:
This study employed ultrahigh-performance liquid chromatograph-hybrid quadrupole orbitrap high resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) to detect the chemical constituents of traditional Chinese medicine in Sizi Dingchuan granules. Network pharmacology and molecular docking techniques were then utilized to predict the effects of Sizi Dingchuan granules on bronchial asthma, along with its target molecules and signaling pathways. UHPLC analysis identified 172 compounds, including 84 flavonoids, 38 organic acids and their derivatives, 22 phenylpropanoids, 16 organic oxygen compounds, 3 phenolic compounds, 2 terpenoids, 2 sugars and glycosides, and 5 other compounds. Typical compound fragmentation patterns were documented. Using network pharmacology methods, 117 compounds were ultimately selected for target prediction, establishing a “drug-component-target-disease” network, which revealed that quercetin, schisandrin A, baicalin, gomisin H, and baicalin exhibit multiple targets related to bronchial asthma, suggesting that they may be the active components responsible for the compound’s efficacy in treating this condition. Protein-protein interaction analysis identified core targets including TNF, IL-6, JAK2, STAT3, BCL2, CASP3, and EGFR, primarily clustered within the JAK-STAT signaling pathway. This suggests that the main components of Sizi Dingchuan granules may regulate the balance between Th17 and Treg cells, as well as Th1 and Th2 cell differentiation, by modulating the JAK-STAT signaling pathway, thereby exerting a positive therapeutic effect on bronchial asthma.
