Research on the transdermal delivery of triptolide encapsulated in hyaluronic acid-phospholipid micelles for the treatment of psoriasis
10.11665/j.issn.1000-5048.2025051201
- VernacularTitle:透明质酸-磷脂胶束包载雷公藤甲素皮肤给药治疗银屑病的研究
- Author:
Xiaoli WANG
1
;
Xiangyi LIU
;
Xiaohui NING
;
Zhenhai ZHANG
;
Yuling WANG
;
Yu BAO
;
Huixia LYU
;
Peiwei ZHU
Author Information
1. 南京中医药大学附属中西医结合医院, 南京 210028;中医药研究院, 南京 210028;中国药科大学药学院, 南京 210009;富启睿医药研发有限公司, 北京 100020;华中科技大学生命科学与技术学院, 武汉 430074;华熙生物科技股份有限公司, 济南 250101
- Publication Type:Journal Article 期刊文章
- Keywords:
psoriasis;
triptolide;
hyaluronic acid-phospholipid;
polymeric micelles;
skin retention
- From:
Journal of China Pharmaceutical University
2025;56(6):719-728
- CountryChina
- Language:Chinese
-
Abstract:
Psoriasis, a chronic, immune-mediated inflammatory disease characterized by hyperproliferation of keratinocytes, is difficult to cure and prone to relapse, often leading to systemic damage. Triptolide (TPL) can modulate cutaneous immune responses and inflammation, yet its therapeutic window is narrow with significant toxicity. To enhance skin targeting and retention of TPL while reducing systemic absorption and toxicity, a TPL/hyaluronic acid/phospholipid polymeric micelle (TPL/HA-DOPE) was constructed via HA's targeting of the CD44 receptor on skin cells. The prepared TPL/HA-DOPE exhibited a uniform spherical morphology with particle size of (130.4±1.23) nm, drug loading capacity of (19.74±0.084) %, and encapsulation efficiency of (85.53±1.34) %. Transdermal permeation studies in vitro and in vivo demonstrated that TPL/HA-DOPE not only enhanced uptake in HaCaT cells but also exhibited excellent skin retention. In a murine model of psoriasis, the TPL/HA-DOPE gel at the dose of 50 μg/(kg•d) showed the most significant improvement in erythema, scaling, and epidermal thickening. Histological analysis confirmed that TPL/HA-DOPE markedly reduced stratum corneum thickness, epidermal hyperplasia, and inflammatory cell infiltration. Ki67 immunostaining proved that its anti-inflammatory mechanism might be achieved by reducing the number of Ki67-positive cells and lowering the levels of inflammatory factors IL-6 and TNF-α. The above results demonstrate that HA-DOPE as a drug delivery carrier for the treatment of psoriasis-like skin diseases has high value of scientific research and good prospects for clinical application.
