Research on microscopic histochemical localization of Astragalus membranaceus and its application
10.11665/j.issn.1000-5048.2025032001
- VernacularTitle:黄芪显微组织化学定位研究与应用
- Author:
Yong HUANG
1
;
Shengchao SHI
;
Jian ZHANG
;
Chaofeng ZHANG
Author Information
1. 中国药科大学中药学院, 江苏省中药评价与转化创新重点实验室, 南京 211198;中国药科大学中药学院,中日天然药物健康品研究联合实验室, 南京 211198;中国药科大学中药学院,固阳黄芪大健康产品联合实验室, 南京 211198
- Publication Type:Journal Article 期刊文章
- Keywords:
Astragalus membranaceus;
histochemical localization;
saponin;
flavonoid;
astragaloside IV
- From:
Journal of China Pharmaceutical University
2025;56(5):566-571
- CountryChina
- Language:Chinese
-
Abstract:
This study compared the chemical composition differences among various root tissues of Astragalus membranaceus through optimization of histochemical localization methods, thereby providing some reference for targeted extraction and enrichment of bioactive components. The chloral hydrate permeabilization technique was enhanced with sodium nitrite-aluminum nitrate-sodium hydroxide reagent for flavonoid staining. Comparative analyses of total saponins, total flavonoids, and astragaloside IV content in xylem, phloem, and periderm tissues were performed using ultraviolet-visible spectroscopy (UV-Vis) and high-performance liquid chromatography (HPLC). By optimizing staining protocols, interference from natural pigmentation was successfully eliminated, thereby enhancing the visualization of tissue-specific chemical distribution. Quantitative analysis revealed that the phloem contained the highest total saponins (6.92%), while the periderm exhibited peak total flavonoids (0.876%) and exclusive enrichment of astragaloside IV (0.850%). The refined histochemical localization method enables precise characterization of phytochemical distribution across root tissues, which can offer guidance for selective extraction and enrichment strategies, and comprehensively promote the utilization and development of Astragalus membranaceus resources.
