Sodium propionate is superior to mixed short-chain fatty acids in alleviating colitis in mice induced by Citrobacter rodentium
10.11665/j.issn.1000-5048.2024052301
- VernacularTitle:丙酸钠缓解鼠柠檬酸杆菌诱导的小鼠结肠炎效果优于混合短链脂肪酸
- Author:
Yingying LI
1
;
Liuyi WANG
;
Sijin WANG
;
Yiwen YANG
;
Fei XIN
;
Li LI
;
Qiaorong JI
Author Information
1. 徐州医科大学, 徐州 221009;滕州市中心人民医院, 滕州 277500;徐州医科大学基础医学院病理生理学教研室, 徐州 221009;徐州医科大学基础医学院临床与实验病理学实验室, 徐州 221009
- Publication Type:Journal Article 期刊文章
- Keywords:
sodium propionate;
short-chain fatty acids;
Citrobacter rodentium;
colitis
- From:
Journal of China Pharmaceutical University
2025;56(4):488-497
- CountryChina
- Language:Chinese
-
Abstract:
To explore the effect and mechanism of sodium propionate and mixed short-chain fatty acids on colitis induced by Citrobacter rodentium (C.r.) in mice. Mice were induced by oral gavage of C.r. The C.r. growth monitoring, histopathological analysis, qPCR analysis, intestinal permeability test and flow cytometry was used to study the effects of sodium propionate and mixed short-chain fatty acids on intestinal infection. The results showed that sodium propionate could inhibit the growth of C.r. more effectively than mixed short-chain fatty acids. Results of animal experiments showed that sodium propionate significantly reduced the weight loss and intestinal bacterial output in mice. Meanwhile, compared with mixed short chain fatty acids, sodium propionate effectively alleviated the pathological manifestations of colonic inflammatory infiltration, destruction of epithelial cell structure and decrease of goblet cell caused by C.r. infection, also increased the levels of antimicrobial peptides like interleukin-17 (IL-17) and regenerated islet derived protein 3γ (Reg3γ). In addition, sodium propionate decreased intestinal permeability better than mixed short-chain fatty acids, and sodium propionate significantly induced T helper cells 17 (Th17) and regulatory T cells (Treg) differentiation. The results showed that sodium propionate significantly alleviated colitis induced by C.r. infection compared with mixed short-chain fatty acids, which may be related to its inhibition of C.r. growth and enhancement of intestinal anti-infective function. The expected results can provide a safer and effective treatment strategy and scientific basis for colitis.
