In Vitro and In Vivo Chemical Composition Analysis of Reference Sample of Jinshui Liujunjian Based on UPLC-Q-TOF-MS/MS
10.13422/j.cnki.syfjx.20251664
- VernacularTitle:基于UPLC-Q-TOF-MS/MS的经典名方金水六君煎基准样品体内外化学成分分析
- Author:
Xinyue YANG
1
;
Huiyu LI
2
;
Yaqi LOU
1
;
Xingxing WANG
2
;
Guifang YU
1
;
Chenfeng ZHANG
2
;
Zhenzhong WANG
1
Author Information
1. State Key Laboratory of Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture（Jiangsu Kanion Pharmaceutical Co. Ltd.Nanjing University of Chinese Medicine）， Nanjing 210000，China
2. Jiangsu Key Laboratory on Innovation for Marine Medicine and Modern Chinese Medicine，Jangsu Kanion Pharmaceutical Co. Ltd.，Lianyungang 222047，China
- Publication Type:Journal Article
- Keywords:
famous classical formulas;
Jinshui Liujunjian;
component analysis;
substance basis;
ultra performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry（UPLC-Q-TOF-MS/MS）
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2026;32(3):166-173
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo elucidate the chemical composition of the reference sample of Jinshui Liujunjian and its distribution characteristics in blood and tissues of rats. MethodsUltra performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry（UPLC-Q-TOF-MS/MS） was used to detect the reference sample solution， plasma， and tissue samples of Jinshui Liujunjian under positive and negative ion modes， respectively. Qualitative Analysis 10.0 software and a self-constructed database were employed for primary mass spectrum matching.Compound identification was further validated by comparing retention times， secondary mass spectral fragments， reference standards， and literature data to deduce fragmentation pathways. ResultsA total of 122 compounds were identified in the reference sample of Jinshui Liujunjian， including 47 flavonoids， 5 amino acids， 13 iridoids， 16 triterpenoid saponins， etc.， of which 42 compounds were confirmed by comparison with reference substances. A total of 21 prototype components were identified in blood components； 50 prototype components were identified in different tissues， among which 13， 10， 7， 21， 11， 6， 14， and 40 prototype components were identified in the heart， liver， spleen， lung， kidney， brain， large intestine， and stomach， respectively. Among them， 7 compounds such as ferulic acid， glycyrrhizic acid， and nobiletin were exposed in the target organs of lung and kidney. ConclusionThis study elucidates the material basis of the reference samples of Jinshui Liujunjian， primarily composed of flavonoids and triterpenoid saponins， along with their in vivo distribution characteristics. These findings provide a scientific basis for establishing quality evaluation indicators and offer references for subsequent pharmacodynamic and pharmacokinetic investigations.
