Strategies to prevent excessive red blood cells during platelet-rich plasma collection in patients with elevated hematocrit
10.13303/j.cjbt.issn.1004-549x.2025.12.015
- VernacularTitle:高Hct患者行PRP采集时避免红细胞超标措施及效果分析
- Author:
Lijuan YANG
1
;
Qiang TAN
1
;
Ling WU
1
;
Tao PENG
1
;
Xinyu GAN
1
;
Lina REN
1
;
Xin MA
1
Author Information
1. Department of Blood Transfusion, the General Hospital of Western Theater Command, Chengdu 610083, China
- Publication Type:Journal Article
- Keywords:
hematocrit;
platelet-rich plasma (PRP);
apheresis;
red blood cells contamination
- From:
Chinese Journal of Blood Transfusion
2025;38(12):1747-1751
- CountryChina
- Language:Chinese
-
Abstract:
Objective: For patients with elevated hematocrit (Hct), platelet-rich plasma (PRP) apheresis is prone to red blood cell contamination—commonly referred to as “flushing” or erythrocyte carryover—which compromises product quality and therapeutic efficacy. This study reports two clinicaly derived measures to mitigate this issue. Methods: For 21 patients with Hct ≥53%, intravenous 0.9% sodium chloride infusion before apheresis process (replacement method, n=13) or 0.9% sodium chloride fluids hemodilution within the centrifuge bowl during PRP apheresis process (dilution method, n=8) were given, respectively. The collection time, adverse reactions, and the celluar composition of PRP—including white blood cells, red blood cells, and platelet counts—were recorded and compared. Results: Neither method resulted in visible RBC contamination (“flushing”). The red blood cell counts [(0.021±0.014)×10
/L vs (0.019±0.011)×10
/L, P>0.05], white blood cell counts [(2.258±3.288) ×10
/L vs (0.557 5±1.203) ×10
/L, P>0.05], and platelet counts [(1 140±308.2)×10
/L vs (1 105±309.9)×10
/L, P>0.05] in the PRP products obtained by two methods all met the control standards of PRP. There was no significant difference [(2.268±0.927) vs (2.438±0.762) mL/min, P=0.669 2] between the two methods in terms of the speed of PRP collection. One case of adverse reaction occurred with the fluid replacement method, while no adverse reaction occurred with the dilution method. Conclusion: For patients with elevated Hct, both fluid replacement and dilution methods can effectively prevent RBC contamination during PRP collection, yielding products that meet clinical quality standards.
